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Team:Paris/Freezer Digestion Products
From 2009.igem.org
(Difference between revisions)
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|style="background:#FFFF00"| D1 | |style="background:#FFFF00"| D1 | ||
| P3 | | P3 | ||
| - | | BBa-J32015 | + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P3]]BBa-J32015 |
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Line 65: | Line 65: | ||
|style="background:#FFFF00"| D2 | |style="background:#FFFF00"| D2 | ||
| P4 | | P4 | ||
| - | | BBa-B0014 | + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P4]]BBa-B0014 |
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Line 76: | Line 76: | ||
|style="background:#FFFF00"| D3 | |style="background:#FFFF00"| D3 | ||
| P5 | | P5 | ||
| - | | BBa-I14032 | + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P5]]BBa-I14032 |
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Line 87: | Line 87: | ||
|style="background:#FFFF00"| D4 | |style="background:#FFFF00"| D4 | ||
| P7 | | P7 | ||
| - | | Bba-R0040 | + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P7]]Bba-R0040 |
| XbaI | | XbaI | ||
| PstI | | PstI | ||
| Line 98: | Line 98: | ||
|style="background:#FFFF00"| D5 | |style="background:#FFFF00"| D5 | ||
| P8 | | P8 | ||
| - | | BBa-I0500 | + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids P8]]BBa-I0500 |
| XbaI | | XbaI | ||
| PstI | | PstI | ||
Revision as of 14:54, 5 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enz. 1 | Enz. 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | Size | PelB leader sequence | ??? | ??? | 08/04 |
| D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | Size | Double terminator bidirectionnal (B0012-B0011) | ??? | ??? | 08/04 |
| D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | Size | pLac | ??? | ??? | 08/04 |
| D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | Size | TetR repressible promote | ??? | ??? | 08/04 |
| D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | Size | pBad/araC | ??? | ??? | 08/04 |
| D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | ??? | ??? | 08/04 |
| D7 | ??? | ??? | XbaI | PstI | Size | ??? | ??? | ??? | 08/04 |
