Team:Paris/6 August 2009

From 2009.igem.org

(Difference between revisions)
(→Lab work)
(→Lab work)
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**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A1]] = Tg3p = 274bp (utiliser le ladder 100bp)
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A1]] = Tg3p = 274bp (utiliser le ladder 100bp)
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A2]] = TE3 = 1036bp
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A2]] = TE3 = 1036bp
-
***[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A1]] and [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A2]] didn't work
 
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A3]] = ClyA (with RBS and poly G linker = 987bp
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A3]] = ClyA (with RBS and poly G linker = 987bp
**[for more detail[https://2009.igem.org/Team:Paris/Freezer_PCR_products]]
**[for more detail[https://2009.igem.org/Team:Paris/Freezer_PCR_products]]
<center>[[Image:KR000634.JPG]][[Image:A3coupe.JPG]]</center>
<center>[[Image:KR000634.JPG]][[Image:A3coupe.JPG]]</center>
 +
***[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A1]] and [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A2]] didn't work we don't see it on the gel because it's not in E.Coli.
*[Ladder 1kb|nothing|[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]|nothing|[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]|nothing] using 1% agarose during 30min
*[Ladder 1kb|nothing|[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]|nothing|[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]|nothing] using 1% agarose during 30min
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]=TolRII=279bp[for more detail[https://2009.igem.org/Team:Paris/Freezer_PCR_products]]
**[[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]]=TolRII=279bp[for more detail[https://2009.igem.org/Team:Paris/Freezer_PCR_products]]
Line 47: Line 47:
Purification and Digestion
Purification and Digestion
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
</div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px">
-
*Purification of [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A3]], [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A4]], [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]], [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A6]]
+
*Purification of [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A3]], [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A4]]directly in micro-column, [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A5]], [[https://2009.igem.org/Team:Paris/Freezer_PCR_products A6]]directly in micro-column
*Digestion with XbaI and PSTI
*Digestion with XbaI and PSTI
</div>
</div>

Revision as of 14:06, 7 August 2009

NoteBook

June
MTWTFSS
[http://2009.igem.org/Team:Paris/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12] [http://2009.igem.org/Team:Paris/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22] [http://2009.igem.org/Team:Paris/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Paris/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2] [http://2009.igem.org/Team:Paris/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Paris/13_July_2009 13] [http://2009.igem.org/Team:Paris/14_July_2009 14] [http://2009.igem.org/Team:Paris/15_July_2009 15] [http://2009.igem.org/Team:Paris/16_July_2009 16] [http://2009.igem.org/Team:Paris/17_July_2009 17] [http://2009.igem.org/Team:Paris/18_July_2009 18] [http://2009.igem.org/Team:Paris/19_July_2009 19]
[http://2009.igem.org/Team:Paris/20_July_2009 20] [http://2009.igem.org/Team:Paris/21_July_2009 21] [http://2009.igem.org/Team:Paris/22_July_2009 22] [http://2009.igem.org/Team:Paris/23_July_2009 23] [http://2009.igem.org/Team:Paris/24_July_2009 24] [http://2009.igem.org/Team:Paris/25_July_2009 25] [http://2009.igem.org/Team:Paris/26_July_2009 26]
[http://2009.igem.org/Team:Paris/27_July_2009 27] [http://2009.igem.org/Team:Paris/28_July_2009 28] [http://2009.igem.org/Team:Paris/29_July_2009 29] [http://2009.igem.org/Team:Paris/30_July_2009 30] [http://2009.igem.org/Team:Paris/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/Team:Paris/1_August_2009 1] [http://2009.igem.org/Team:Paris/2_August_2009 2]
[http://2009.igem.org/Team:Paris/3_August_2009 3] [http://2009.igem.org/Team:Paris/4_August_2009 4] [http://2009.igem.org/Team:Paris/5_August_2009 5] [http://2009.igem.org/Team:Paris/6_August_2009 6] [http://2009.igem.org/Team:Paris/7_August_2009 7] [http://2009.igem.org/Team:Paris/8_August_2009 8] [http://2009.igem.org/Team:Paris/9_August_2009 9]
[http://2009.igem.org/Team:Paris/10_August_2009 10] [http://2009.igem.org/Team:Paris/11_August_2009 11] [http://2009.igem.org/Team:Paris/12_August_2009 12] [http://2009.igem.org/Team:Paris/13_August_2009 13] [http://2009.igem.org/Team:Paris/14_August_2009 14] [http://2009.igem.org/Team:Paris/15_August_2009 15] [http://2009.igem.org/Team:Paris/16_August_2009 16]
[http://2009.igem.org/Team:Paris/17_August_2009 17] [http://2009.igem.org/Team:Paris/18_August_2009 18] [http://2009.igem.org/Team:Paris/19_August_2009 19] [http://2009.igem.org/Team:Paris/20_August_2009 20] [http://2009.igem.org/Team:Paris/21_August_2009 21] [http://2009.igem.org/Team:Paris/22_August_2009 22] [http://2009.igem.org/Team:Paris/23_August_2009 23]
[http://2009.igem.org/Team:Paris/24_August_2009 24] [http://2009.igem.org/Team:Paris/25_August_2009 25] [http://2009.igem.org/Team:Paris/26_August_2009 26] [http://2009.igem.org/Team:Paris/27_August_2009 27] [http://2009.igem.org/Team:Paris/28_August_2009 28] [http://2009.igem.org/Team:Paris/29_August_2009 29] [http://2009.igem.org/Team:Paris/30_August_2009 30]
[http://2009.igem.org/Team:Paris/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Paris/1_September_2009 1] [http://2009.igem.org/Team:Paris/2_September_2009 2] [http://2009.igem.org/Team:Paris/3_September_2009 3] [http://2009.igem.org/Team:Paris/4_September_2009 4] [http://2009.igem.org/Team:Paris/5_September_2009 5] [http://2009.igem.org/Team:Paris/6_September_2009 6]
[http://2009.igem.org/Team:Paris/7_September_2009 7] [http://2009.igem.org/Team:Paris/8_September_2009 8] [http://2009.igem.org/Team:Paris/9_September_2009 9] [http://2009.igem.org/Team:Paris/10_September_2009 10] [http://2009.igem.org/Team:Paris/11_September_2009 11] [http://2009.igem.org/Team:Paris/12_September_2009 12] [http://2009.igem.org/Team:Paris/13_September_2009 13]
[http://2009.igem.org/Team:Paris/14_September_2009 14] [http://2009.igem.org/Team:Paris/15_September_2009 15] [http://2009.igem.org/Team:Paris/16_September_2009 16] [http://2009.igem.org/Team:Paris/17_September_2009 17] [http://2009.igem.org/Team:Paris/18_September_2009 18] [http://2009.igem.org/Team:Paris/19_September_2009 19] [http://2009.igem.org/Team:Paris/20_September_2009 20]
[http://2009.igem.org/Team:Paris/21_September_2009 21] [http://2009.igem.org/Team:Paris/22_September_2009 22] [http://2009.igem.org/Team:Paris/23_September_2009 23] [http://2009.igem.org/Team:Paris/24_September_2009 24] [http://2009.igem.org/Team:Paris/25_September_2009 25] [http://2009.igem.org/Team:Paris/26_September_2009 26] [http://2009.igem.org/Team:Paris/27_September_2009 27]
[http://2009.igem.org/Team:Paris/28_September_2009 28] [http://2009.igem.org/Team:Paris/29_September_2009 29] [http://2009.igem.org/Team:Paris/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Paris/1_October_2009 1] [http://2009.igem.org/Team:Paris/2_October_2009 2] [http://2009.igem.org/Team:Paris/3_October_2009 3] [http://2009.igem.org/Team:Paris/4_October_2009 4]
[http://2009.igem.org/Team:Paris/5_October_2009 5] [http://2009.igem.org/Team:Paris/6_October_2009 6] [http://2009.igem.org/Team:Paris/7_October_2009 7] [http://2009.igem.org/Team:Paris/8_October_2009 8] [http://2009.igem.org/Team:Paris/9_October_2009 9] [http://2009.igem.org/Team:Paris/10_October_2009 10] [http://2009.igem.org/Team:Paris/11_October_2009 11]
[http://2009.igem.org/Team:Paris/12_October_2009 12] [http://2009.igem.org/Team:Paris/13_October_2009 13] [http://2009.igem.org/Team:Paris/14_October_2009 14] [http://2009.igem.org/Team:Paris/15_October_2009 15] [http://2009.igem.org/Team:Paris/16_October_2009 16] [http://2009.igem.org/Team:Paris/17_October_2009 17] [http://2009.igem.org/Team:Paris/18_October_2009 18]
[http://2009.igem.org/Team:Paris/19_October_2009 19] [http://2009.igem.org/Team:Paris/20_October_2009 20] [http://2009.igem.org/Team:Paris/21_October_2009 21] [http://2009.igem.org/Team:Paris/22_October_2009 22] [http://2009.igem.org/Team:Paris/23_October_2009 23] [http://2009.igem.org/Team:Paris/24_October_2009 24] [http://2009.igem.org/Team:Paris/25_October_2009 25]
[http://2009.igem.org/Team:Paris/26_October_2009 26] [http://2009.igem.org/Team:Paris/27_October_2009 27] [http://2009.igem.org/Team:Paris/28_October_2009 28] [http://2009.igem.org/Team:Paris/29_October_2009 29] [http://2009.igem.org/Team:Paris/30_October_2009 30] [http://2009.igem.org/Team:Paris/31_October_2009 31]

← Yesterday - Tomorrow →

August 6th

Lab work

FM4-64 dying MG4 and Kayo

protocol n°2 (05/08/09) but using PBS

->nearly the same as MgSO4

protocol n°2 (05/08/09)but 1ml of cells and 1µl of dye, one with MgSO4 and one with PBS

->not enough dye

Gel Migration

  • [Ladder 1kb|[A1]|nothing|[A2]|nothing|[A3]]using 1% agarose during 30min
    • [A1] = Tg3p = 274bp (utiliser le ladder 100bp)
    • [A2] = TE3 = 1036bp
    • [A3] = ClyA (with RBS and poly G linker = 987bp
    • [for more detail[1]]
KR000634.JPGA3coupe.JPG
      • [A1] and [A2] didn't work we don't see it on the gel because it's not in E.Coli.
  • [Ladder 1kb|nothing|[A5]|nothing|[A5]|nothing] using 1% agarose during 30min
    • [A5]=TolRII=279bp[for more detail[2]]
KR000637.JPGA5coupé.JPG

Purification and Digestion

  • Purification of [A3], [A4]directly in micro-column, [A5], [A6]directly in micro-column
  • Digestion with XbaI and PSTI

Writing tomorow...

  • Nouvelles Miniprep pour P1(pSB2K3), P13(pSB1A3), P14(BBa_K136050) => Nulles à refaire pousser ON et miniprep a refaire demain
  • ON pour S8(pSB2K3), S29(pSB1A3), S30(BBa_K136050)
  • Digestion P1(pSB2K3), P2(BBa_J61002), P7(Bba_R0040) 2h37 par respectivement XP, XS, XP
  • Migration sur gel pour verifier 1kb, P1, P2, P7, 100bp
  • Transformation 1L7 BBa_J24679 AmpR+ dans DH5a

To do list

Matricule TODO
Luc Labs
Romain Look for his feet
Charlotte Jun/Fos system (oligo + system)
Stoff DataBase / kitchen system ?
Chris modeling: model genetique network / next week lab planning / gillepsy
Lisa WTF !!!
Caroline lab / microscope
Souf lab / oligo / wiki
Vicard Lab : Gel :D
Pierre Tol/pal modeling
Sylvain if(!oligo){return Maltose;} return PCR;
Guillaume ??


← Yesterday - Tomorrow →

Retrieved from "http://2009.igem.org/Team:Paris/6_August_2009"