Team:Paris/Fridge
From 2009.igem.org
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! colspan="10" style="background: #ccccff;"|Fridge tube | ! colspan="10" style="background: #ccccff;"|Fridge tube | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
- | |width=5%| | + | |width=5%| Ref |
- | |width= | + | |width=40%| Name |
- | |width= | + | |width=40%| Comments |
|width=5%| Quantity | |width=5%| Quantity | ||
+ | |width=5%| Storage | ||
|width=5%| Date | |width=5%| Date | ||
|- style="background: #ccccff; text-align: left;" | |- style="background: #ccccff; text-align: left;" | ||
|F1 | |F1 | ||
- | |[[https://2009.igem.org/Team:Paris/ | + | |[[https://2009.igem.org/Team:Paris/Freezer_Strains_%28Glycerol_stock%29#Freezer#Strain_1 S1]] DH5alpha |
|Overnight culture for Glycerol Stock | |Overnight culture for Glycerol Stock | ||
+ | |Ø | ||
| | | | ||
|[[Team:Paris/21_July_2009 | 07/21]] | |[[Team:Paris/21_July_2009 | 07/21]] | ||
Line 26: | Line 28: | ||
|[[https://2009.igem.org/Team:Paris/Strains#Strain_2 S2]] K12 MG 1655 delta recA::Kan | |[[https://2009.igem.org/Team:Paris/Strains#Strain_2 S2]] K12 MG 1655 delta recA::Kan | ||
|Overnight culture for Glycerol Stock | |Overnight culture for Glycerol Stock | ||
+ | |Ø | ||
| | | | ||
|[[Team:Paris/22_July_2009 | 07/21]] | |[[Team:Paris/22_July_2009 | 07/21]] | ||
Line 33: | Line 36: | ||
|[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha | |[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha | ||
|Overnight culture for competent bacteria | |Overnight culture for competent bacteria | ||
+ | |Ø | ||
| | | | ||
|[[Team:Paris/22_July_2009 | 07/22]] | |[[Team:Paris/22_July_2009 | 07/22]] | ||
Line 41: | Line 45: | ||
|Genomic DNA Purification | |Genomic DNA Purification | ||
| | | | ||
+ | |4°C | ||
|[[Team:Paris/22_July_2009 | 07/22]] | |[[Team:Paris/22_July_2009 | 07/22]] | ||
Line 48: | Line 53: | ||
|Filtred the 07/23 | |Filtred the 07/23 | ||
|400ml | |400ml | ||
+ | |4°C | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
Line 55: | Line 61: | ||
|Filtred the 07/23 | |Filtred the 07/23 | ||
|25ml | |25ml | ||
+ | |4°C | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
Line 62: | Line 69: | ||
|Filtred the 07/23 | |Filtred the 07/23 | ||
|25ml | |25ml | ||
+ | |4°C | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
Line 69: | Line 77: | ||
|Filtred the 07/23 | |Filtred the 07/23 | ||
|25ml | |25ml | ||
+ | |4°C | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
Line 75: | Line 84: | ||
|CaCl<sub>2</sub> 0,1M | |CaCl<sub>2</sub> 0,1M | ||
|Filtred the 07/23 | |Filtred the 07/23 | ||
- | | | + | |5ml |
+ | |4°C | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
Line 82: | Line 92: | ||
|[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha | |[[https://2009.igem.org/Team:Paris/Strains#Strain_1 S1]] DH5alpha | ||
|Overnight culture for competent bacteria | |Overnight culture for competent bacteria | ||
+ | |Ø | ||
| | | | ||
|[[Team:Paris/23_July_2009 | 07/23]] | |[[Team:Paris/23_July_2009 | 07/23]] | ||
+ | |||
+ | |- style="background: #ccccff; text-align: left;" | ||
+ | |F7 | ||
+ | |CaCl<sub>2</sub> 50mM | ||
+ | |Filtred the 07/23 | ||
+ | |18ml | ||
+ | |4°C | ||
+ | |[[Team:Paris/23_July_2009 | 07/23]] | ||
+ | |||
+ | |- style="background: #ccccff; text-align: left;" | ||
+ | |F8 | ||
+ | |DH5 alpha competent | ||
+ | |by CaCl<sub>2</sub> | ||
+ | |2ml | ||
+ | |4°C | ||
+ | |[[Team:Paris/23_July_2009 | 07/23]] | ||
+ | |||
+ | |- style="background: #ccccff; text-align: left;" | ||
+ | |F9 | ||
+ | |Buffer I | ||
+ | |For SuperCompetent Bacteria Protocol | ||
+ | |250ml | ||
+ | |4°C | ||
+ | |[[Team:Paris/27_July_2009 | 07/27]] | ||
+ | |||
+ | |- style="background: #ccccff; text-align: left;" | ||
+ | |F10 | ||
+ | |Buffer II | ||
+ | |For SuperCompetent Bacteria Protocol | ||
+ | |125ml | ||
+ | |4°C | ||
+ | |[[Team:Paris/27_July_2009 | 07/27]] | ||
|- style="background: #ccccff; text-align: left;" | |- style="background: #ccccff; text-align: left;" | ||
Line 90: | Line 133: | ||
|x | |x | ||
| | | | ||
+ | |4°C | ||
|[[Team:Paris/xx_July_2009 | 07/xx]] | |[[Team:Paris/xx_July_2009 | 07/xx]] | ||
|} | |} |
Latest revision as of 13:13, 9 August 2009
Fridge tube
Fridge tube | |||||||||
---|---|---|---|---|---|---|---|---|---|
Ref | Name | Comments | Quantity | Storage | Date | ||||
F1 | [S1] DH5alpha | Overnight culture for Glycerol Stock | Ø | 07/21 | |||||
F2 | [S2] K12 MG 1655 delta recA::Kan | Overnight culture for Glycerol Stock | Ø | 07/21 | |||||
F3 | [S1] DH5alpha | Overnight culture for competent bacteria | Ø | 07/22 | |||||
F4 | Genomic DNA of [S2] K12 MG 1655 delta recA::Kan | Genomic DNA Purification | 4°C | 07/22 | |||||
F5 | Stock CaCl2 0,1M | Filtred the 07/23 | 400ml | 4°C | 07/23 | ||||
F5.1 | CaCl2 0,1M | Filtred the 07/23 | 25ml | 4°C | 07/23 | ||||
F5.2 | CaCl2 0,1M | Filtred the 07/23 | 25ml | 4°C | 07/23 | ||||
F5.3 | CaCl2 0,1M | Filtred the 07/23 | 25ml | 4°C | 07/23 | ||||
F5.4 | CaCl2 0,1M | Filtred the 07/23 | 5ml | 4°C | 07/23 | ||||
F6 | [S1] DH5alpha | Overnight culture for competent bacteria | Ø | 07/23 | |||||
F7 | CaCl2 50mM | Filtred the 07/23 | 18ml | 4°C | 07/23 | ||||
F8 | DH5 alpha competent | by CaCl2 | 2ml | 4°C | 07/23 | ||||
F9 | Buffer I | For SuperCompetent Bacteria Protocol | 250ml | 4°C | 07/27 | ||||
F10 | Buffer II | For SuperCompetent Bacteria Protocol | 125ml | 4°C | 07/27 | ||||
Fx | x | x | 4°C | 07/xx |