Team:Paris/9 August 2009
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===Lab work=== | ===Lab work=== | ||
- | + | <div class="guillaume"> | |
+ | ON culture | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | *tri strain (for Caroline) 6ml LB | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | New transformation | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | *pSB2K3 in order to have new clones because I can't get the right digestion profil | ||
+ | **NB: 1h20 of incubation at 37°C under agitation (Miam time --!) | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | PCR | ||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | *For A11 (ex A4) ??name??: Matrix = Genomic DNA of K12, Oligo = O11 and O12 | ||
+ | *For A13 (ex A5) ??name??: Matrix = Genomic DNA of K12, Oligo = O1 and O13 | ||
+ | **1:98°C 30s | ||
+ | **2:98°C 10s | ||
+ | **3:70°C 30s | ||
+ | **4:72°C 8s | ||
+ | **Goto 3 29x | ||
+ | **5:72°C 10min | ||
+ | **6: 4°C ~ | ||
+ | NB:1h at 98°C because I haven't push the "enter" buton after it warm up to 98°C...and all evaporate. | ||
+ | </div> | ||
+ | <div class="guillaume"> | ||
+ | |||
+ | </div> | ||
+ | <div class="experience"> | ||
+ | </div> | ||
<html> | <html> | ||
</div> | </div> |
Revision as of 20:49, 9 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
ON culture
- tri strain (for Caroline) 6ml LB
New transformation
- pSB2K3 in order to have new clones because I can't get the right digestion profil
- NB: 1h20 of incubation at 37°C under agitation (Miam time --!)
PCR
- For A11 (ex A4) ??name??: Matrix = Genomic DNA of K12, Oligo = O11 and O12
- For A13 (ex A5) ??name??: Matrix = Genomic DNA of K12, Oligo = O1 and O13
- 1:98°C 30s
- 2:98°C 10s
- 3:70°C 30s
- 4:72°C 8s
- Goto 3 29x
- 5:72°C 10min
- 6: 4°C ~
NB:1h at 98°C because I haven't push the "enter" buton after it warm up to 98°C...and all evaporate.