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Team:TUDelft/29 July 2009
From 2009.igem.org
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| 14 || pSB1AK3 II || 3864 || <font color=red>✖</font> | | 14 || pSB1AK3 II || 3864 || <font color=red>✖</font> | ||
|- align="center" | |- align="center" | ||
| - | | 15 || λp-GFP I || | + | | 15 || λp-GFP I || 3089 || <font color=limegreen>✔</font> |
|- align="center" | |- align="center" | ||
| - | | 16 || λp-GFP II || | + | | 16 || λp-GFP II || 3089 || <font color=limegreen>✔</font> |
|- align="center" | |- align="center" | ||
| 17 || pLacI || || | | 17 || pLacI || || | ||
Revision as of 09:32, 10 August 2009
Lab Notebook
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29 July 2009
Calin
Checked concentrations from yesterday's miniprep.
| Part | Concentration (ng/uL) |
| pSB1AK3 I | 92 |
| pSB1AK3 II | 77 |
| lambda_p GFP I | 119 |
| lambda_p GFP II | 108 |
| pLacI | 56.7 |
| RBS | 95.2 |
Todays gel:
| Well | Part | Expected Plasmid Size | Status |
| 1 | BBa_K142205 PCR | ||
| 2 | BBa_K142202 PCR | ||
| 3 | BBa_B0015 PCR | ||
| 4 | BBa_R0040 PCR | ||
| 5 | BBa_K145201 PCR | ||
| 6 | *I3 uncut | ||
| 7 | *I3 BglI cut | ||
| 8 | *I3 BglI+I-SceI cut | ||
| 9 | *I6 uncut | ||
| 10 | *I6 BglI cut | ||
| 11 | *I7 BglI+I-SceI cut | ||
| 12 | [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] | ||
| 13 | pSB1AK3 I | 3864 | ✖ |
| 14 | pSB1AK3 II | 3864 | ✖ |
| 15 | λp-GFP I | 3089 | ✔ |
| 16 | λp-GFP II | 3089 | ✔ |
| 17 | pLacI | ||
| 18 | rbs | 2091 | ✔ |
Orr
Got the source code in R for the modelling of conjugation, and already looked at how it works.
