"
Team:Paris/constructions
From 2009.igem.org
(Difference between revisions)
(→Constructions) |
(→Constructions) |
||
| Line 123: | Line 123: | ||
|BBa_???? | |BBa_???? | ||
| + | |- style="background: #FF0000; text-align: center" | ||
| + | |CC | ||
| + | |((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| + | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| + | |modif RFP (souf) | ||
| + | |?? | ||
| + | |BBa_???? | ||
| + | |||
| + | |- style="background: #FF0000; text-align: center" | ||
| + | |CC | ||
| + | |((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| + | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| + | |modif RFP (souf) | ||
| + | |??fusion N term de cly A (autre possibilité) | ||
| + | |BBa_???? | ||
|- style="background: #FF0000; text-align: center" | |- style="background: #FF0000; text-align: center" | ||
|CC! | |CC! | ||
| - | |((rbs - ClyA | + | |((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| - | | | + | |modif RFP (souf) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
| Line 134: | Line 149: | ||
|- style="background: #FF0000; text-align: center" | |- style="background: #FF0000; text-align: center" | ||
|CC! | |CC! | ||
| - | |((rbs - ClyA | + | |((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| - | | | + | |modif RFP (souf) |
|?? | |?? | ||
|BBa_???? | |BBa_???? | ||
| Line 142: | Line 157: | ||
|- style="background: #CC0000; text-align: center" | |- style="background: #CC0000; text-align: center" | ||
|CC! | |CC! | ||
| - | |(Pbad :: ((rbs - ClyA | + | |(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |[http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
| Line 150: | Line 165: | ||
|- style="background: #CC0000; text-align: center" | |- style="background: #CC0000; text-align: center" | ||
|CC! | |CC! | ||
| - | |(Pbad :: ((rbs - ClyA | + | |(Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
| Line 158: | Line 173: | ||
|- style="background: #990000; text-align: center; color:white;" | |- style="background: #990000; text-align: center; color:white;" | ||
|CC! | |CC! | ||
| - | |((Pbad :: ((rbs - ClyA | + | |((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
|2*Pbad et 2*ter?? (souf) | |2*Pbad et 2*ter?? (souf) | ||
| Line 166: | Line 181: | ||
|- style="background: #990000; text-align: center; color:white;" | |- style="background: #990000; text-align: center; color:white;" | ||
|CC! | |CC! | ||
| - | |((Pbad :: ((rbs - ClyA | + | |((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] |
|BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | |BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ||
| | | | ||
Revision as of 03:28, 11 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Constructions
- Plasmid A
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| CC | (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC | (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC | ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
| |
| CC | (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
| |
| CC | (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC | ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
| CC | ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ??fusion N term de cly A (autre possibilité) | BBa_???? |
| CC! | ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
| CC! | ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | modif RFP (souf) | ?? | BBa_???? |
| CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_???? | |
| CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | 2*Pbad et 2*ter?? (souf) | ?? | BBa_???? |
| CC! | ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] | ?? | BBa_????
|
- Plasmid B
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| GC | ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 | BBa_blabla :: BBa_tralala in truc | Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) | 12 | BBa_pouet pouet |
| CC | (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CL | (rbs - OmpAsignal :: TolR II) | BBa_???? | oligo designed, | ?? | BBa_???? |
| CC | (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) | ?? | BBa_???? |
| CC | (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? | |
| CC | ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] | ?? | BBa_???? |
- Plasmid C
| Designer | Parts description | Corresponding BioBricks | Status (last update) | Plasmid # | Registry |
| CC | Pfec in ?? | BBa_??? | ?? | BBa_????
| |
| CC | Pfec in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | BBa_??? | ?? | BBa_???? |
