Team:Paris/constructions

From 2009.igem.org

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(→Constructions)
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|- style="background: #FFCC00; text-align: center"
|- style="background: #FFCC00; text-align: center"
|CC!
|CC!
-
|((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+
|((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
| BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3]
-
|jun/foss has to be synthetized (10/08/09)
+
|jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09)
|??
|??
|BBa_????
|BBa_????

Revision as of 08:22, 11 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Constructions

  • Plasmid A


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (Pbad :: rbs - lacI - LVA) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]???Pbad is on PSB2K3 [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] pSB1A3 transformed, digested, PBad transformed and not digested well- LacI transformed and digested (10/08/09) ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ter transformed, digested and purified (10/08/09) ?? BBa_????
CC ((Pbad :: rbs - lacI - LVA) :: RFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] pSB2K3 transformed and digested, RFP : primers designed but not yet ordered (10/08/09) ?? BBa_????


CC (((Pbad :: rbs - lacI - LVA) :: RFP) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC (OmpA Linker :: g3p) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] OmpA linker standardisé - OmpA amplified, not purified - Same for g3p (10/08/09) ?? BBa_????
(g3p :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] autre possibilité (souf)- 1 primer missing for C term fusion with clyA (10/08/09) ?? BBa_????
CC! ((OmpA Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((???autotransporter??? - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] jun/foss has to be synthetized & autotransporter to be found!!!! (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((rbs - jun/foss) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] FecA : oligo ordered but not received yet (10/08/09) ?? BBa_????
CC! ((rbs - fecA) :: ((Ompa Linker :: g3p) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC ((rbs - ClyA :: RFP) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf)- some troubles with amplification of clyA fusion C term (10/08/09) ?? BBa_????
CC ((rbs-RFP :: clyA) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) - fusion N term de cly A (autre possibilité)- we still miss 1 primer for clyA N term fusion (10/08/09) ?? BBa_????
CC! ((rbs - ClyA :: RFP)) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] modif RFP (souf) ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! (Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] 2*Pbad et 2*ter?? (souf) ?? BBa_????
CC! ((Pbad :: ((rbs - ClyA :: RFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????



  • Plasmid B


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
GC ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 BBa_blabla :: BBa_tralala in truc Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) 12 BBa_pouet pouet
CC (Plac :: rbs-tetR) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] both have been digested and purified, ligation in progress (10/08/09) ?? BBa_????
CC ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_???? :: [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CL (rbs - OmpAsignal :: TolR II) BBa_???? OmpA signal and TolRII amplified, but neither digested nor purified (10/08/09) ?? BBa_????
CC (Ptet :: (rbs - OmpAsignal :: TolRII)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] Ptet digested and purified (10/08/09) ?? BBa_????
CC (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] y a t-il vraiment 2 biobricks ou bien met-on le RBS direct dans des oligos pour RFP? (souf) biobrick to find or primers to design (10/08/09) ?? BBa_????
CC (Ptet :: (rbs :: EYFP)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ((Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter))) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????


  • Plasmid C


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC (rbs-FecI :: rbs-FecR) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? primers ordered but not received yet (10/08/09) ?? BBa_????


CC PfecA in [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] BBa_??? vector digested and purified, Pfec A amplified but not digested (purified?) (10/08/09) ?? BBa_????

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