Team:TUDelft/13 August 2009

From 2009.igem.org

(Difference between revisions)
(→13 August 2009)
(→Calin)
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Knockout day.
Knockout day.
 +
 +
Made 1M l-arabinose stock.
 +
 +
Digested CE with EcoRI (10uL DNA).
Yesterdays gel:
Yesterdays gel:
[[Image:Gel120809-delay-conj.png|600px]]
[[Image:Gel120809-delay-conj.png|600px]]
 +
 +
 +
{| border="1" align="center"
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| Well || Part || Expected Plasmid Size || Status
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|- align="center"
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| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 2 ||  || ||
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|- align="center"
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| 3 ||  || ||
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|- align="center"
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| 4 ||  || ||
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|- align="center"
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| 5 ||  || ||
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|- align="center"
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| 6 ||  || ||
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|- align="center"
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| 7 ||  || ||
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|- align="center"
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| 8 ||  || ||
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|- align="center"
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| 9 ||  ||  ||
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|- align="center"
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| 10 ||  || ||
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|- align="center"
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| 11 ||  || ||
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|- align="center"
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| 12 ||  || ||
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|- align="center"
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| 13 ||  || ||
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|- align="center"
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| 14 ||  || ||
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|- align="center"
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| 15 ||  || ||
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|- align="center"
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| 16 ||  || ||
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|- align="center"
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| 17 || trbK X+P || || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 18 || GFP-gen E+S || || <font color=limegreen>&#10004;</font>
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|- align="center"
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| 19 || pTet+RBS E+S || || ?
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|- align="center"
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| 20 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
 +
|}
 +
 +
Made 12 plates. 4 High KAN. 4 Low KAN. 4 TRI.
 +
 +
Placed 50uL of overnight R751 + pKD46 culture into 4 x 5 mL tubes.
 +
 +
In 30C incubator at 11:15
 +
 +
11:45 0.034 OD
 +
 +
12:30 0.065 OD
 +
 +
13:17 0.126 OD
 +
 +
Induced +L-arabinose tubes with L-arabinose (40uL 1M stock added to 4mL). Control tube has no L-arabinose added.
 +
 +
In 30C incubator at 13:25
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 11:43, 13 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

13 August 2009

Calin

Knockout day.

Made 1M l-arabinose stock.

Digested CE with EcoRI (10uL DNA).

Yesterdays gel:

Gel120809-delay-conj.png


Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17 trbK X+P ✔
18 GFP-gen E+S ✔
19 pTet+RBS E+S  ?
20 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔

Made 12 plates. 4 High KAN. 4 Low KAN. 4 TRI.

Placed 50uL of overnight R751 + pKD46 culture into 4 x 5 mL tubes.

In 30C incubator at 11:15

11:45 0.034 OD

12:30 0.065 OD

13:17 0.126 OD

Induced +L-arabinose tubes with L-arabinose (40uL 1M stock added to 4mL). Control tube has no L-arabinose added.

In 30C incubator at 13:25

Retrieved from "http://2009.igem.org/Team:TUDelft/13_August_2009"