Team:Paris/12 August 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{Template:Paris2009}} {{Template:Paris2009_menu}} ==NoteBook== {| style="color:#1b2c8a;background-color:#0;" cellpadding="0" cellspacing="1" border="0" bordercolor="#fff" width="100%" a...)
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==NoteBook==
==NoteBook==
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{{Paris2009_Calendar}}
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{| style="color:#1b2c8a;background-color:#0;" cellpadding="0" cellspacing="1" border="0" bordercolor="#fff" width="100%" align="left"
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{{Paris2009_Calendar_Link|11_August_2009|13_August_2009}}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=06 }}
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<center> '''August 12th''' </center>
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=07 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=08 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=09 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=10 }}
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|}
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<html>
<html>
</div>
</div>
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==Brain work==
 
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===Lab work===
 +
<div class="vicard">
 +
Ligation
 +
</div>
 +
<div class="experience">
 +
[https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#D15 D15] (2xterminator E/S) and [https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#D16 D16] (pLac E/X) with vector psb2k3
-
edit please ^^
+
DO : D15=1,19µg/mL  D16=1,75µg/mL
 +
{|
 +
|- style="background: #b3d0E4; text-align: center; color:black; "
 +
|width=10%|
 +
|width=10%|
 +
|width=10%| vecteur
 +
|width=10%| insert
 +
|width=10%| H20
 +
|width=10%| Buffer 10x T4 ligase
 +
|width=10%| T4 ligase
 +
|width=10%| Total
 +
 +
|- style="background: #E0E9EF; text-align: center;"
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|width=10%| L5
 +
|width=10%| 2xterm:pSB2K3(with pLac)
 +
|width=10%| 1µl
 +
|width=10%| 3µl
 +
|width=10%| 13µl
 +
|width=10%| 2µl
 +
|width=10%| 1µl
 +
|width=10%| 20µl
 +
 +
|- style="background: #E0E9EF; text-align: center;"
 +
|width=10%| control -
 +
|width=10%| o
 +
|width=10%| 1µl
 +
|width=10%| o
 +
|width=10%| 15µl
 +
|width=10%| 2µl
 +
|width=10%| 1µl
 +
|width=10%| 20µl
 +
|}
 +
 +
 +
1h Home temperature
-
<html>
 
</div>
</div>
-
<div id="paris_content_boxtop">
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 +
<div class="vicard">
 +
Transformation
</div>
</div>
-
<div id="paris_content">
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<div class="experience">
-
</html>
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classic protocol with DH5a
 +
</div>
-
==Lab work==
+
<div class="vicard">
 +
Purification
 +
</div>
 +
<div class="experience">
 +
*Purification of A10(ClyA) (PCR at 55° during 30sec) (using 1% agarose during 25 min)
 +
*A10=[https://2009.igem.org/Team:Paris/Freezer_PCR_products#Freezer#A10 A10] 987pb
 +
<center>[[Image:ClyA_pcr2.JPG]]</center>
 +
<center>[[Image:Cly1_pcr3.JPG]]</center>
 +
<center>[[Image:ClyA_coupé.JPG]]</center>
 +
*A10 purificated
 +
<center>[[Image:ClyA_purif.JPG]]</center>
 +
*Purification of A10 work
 +
</div>
-
edit please ^^
+
<div class="sylvain">
 +
PCR Gel verification
 +
</div>
 +
<div class="experience">
 +
1% aga gel after PCR under different conditions for A18 (OmpA-Linker from Varsaw).
 +
Expected weight : 525bp.
 +
<center>[[Image:Gel_090812Sylvain.JPG]]</center>
 +
Obviously, something has gone wrong. Next step will be different temperature gradients testing for PCR and/or new matrix.
 +
</div>
 +
<div class="sylvain">
 +
Transformation
 +
</div>
 +
<div class="experience">
 +
Classic protocol with DH5a, and pSB2K3 from iGEM DNA plates (1/7C). ON culture on LB + Kan.
 +
 +
To be checked tomorrow !
 +
</div>
 +
 +
<div class="sylvain">
 +
ON culture for Minipreps tomorrow
 +
</div>
 +
<div class="experience">
 +
pSB1A3, pLAC, RBSTetR, OmpA-L, Three ligation clones, ON culture in LB + Abiotics. 37°C with stiring.
 +
</div>
 +
 +
<div class="sylvain">
 +
ON PCR
 +
</div>
 +
<div class="experience">
 +
A20 : 49, 51 and 53°C PCR with the plasmid for OmpA-L. Overnigth. 15s elongation time.
 +
</div>
 +
 +
<div class="guillaume">
 +
All Manip
 +
</div>
 +
<div class="experience">
 +
Comming soon
 +
</div>
<html>
<html>
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</html>
</html>
 +
===To do list===
 +
 +
{|
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=100px| Matricule
 +
|width=800px| TODO
 +
|- style="background: #59eb26;text-align: center;"
 +
|Luc
 +
|
 +
|- style="background: #efe715;text-align: center;"
 +
|Romain
 +
|
 +
|- style="background: #d8279c;text-align: center;"
 +
|Charlotte
 +
|
 +
|- style="background: #fbfbfb;text-align: center;"
 +
|Stoff
 +
|
 +
|- style="background: #0824f5;text-align: center;"
 +
|Chris
 +
| 
 +
|- style="background: #06ff00;text-align: center;"
 +
|Lisa
 +
|
 +
|- style="background: #b40ecd;text-align: center;"
 +
|Caroline
 +
|
 +
|- style="background: #ff9966;text-align: center;"
 +
|Souf
 +
|
 +
|- style="background: #79f4f8;text-align: center;"
 +
|Vicard
 +
|
 +
|- style="background: #e35050;text-align: center;"
 +
|Pierre
 +
|
 +
|- style="background: #bababa;text-align: center;"
 +
|Sylvain
 +
|pSB2K3 transformation ; PCR gels and purifs or new culture for minipreps
 +
|- style="background: #009900;text-align: center;"
 +
|Guillaume
 +
|
 +
|}
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==To do list==
 
-
edit please ^^
+
{{Paris2009_Calendar_Link|11_August_2009|13_August_2009}}

Latest revision as of 22:37, 16 August 2009

NoteBook

June
MTWTFSS
[http://2009.igem.org/Team:Paris/1_June_2009 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Paris/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Paris/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_June_2009&action=edit 12] [http://2009.igem.org/Team:Paris/13_June_2009 13] [http://2009.igem.org/wiki/index.php?title=Team:Paris/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Paris/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Paris/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Paris/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Paris/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Paris/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Paris/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/22_June_2009&action=edit 22] [http://2009.igem.org/Team:Paris/23_June_2009 23] [http://2009.igem.org/wiki/index.php?title=Team:Paris/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Paris/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Paris/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Paris/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Paris/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Paris/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Team:Paris/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Paris/2_July_2009&action=edit 2] [http://2009.igem.org/Team:Paris/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Paris/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Paris/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Team:Paris/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Paris/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Paris/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Paris/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Paris/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Paris/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Paris/12_July_2009&action=edit 12]
[http://2009.igem.org/Team:Paris/13_July_2009 13] [http://2009.igem.org/Team:Paris/14_July_2009 14] [http://2009.igem.org/Team:Paris/15_July_2009 15] [http://2009.igem.org/Team:Paris/16_July_2009 16] [http://2009.igem.org/Team:Paris/17_July_2009 17] [http://2009.igem.org/Team:Paris/18_July_2009 18] [http://2009.igem.org/Team:Paris/19_July_2009 19]
[http://2009.igem.org/Team:Paris/20_July_2009 20] [http://2009.igem.org/Team:Paris/21_July_2009 21] [http://2009.igem.org/Team:Paris/22_July_2009 22] [http://2009.igem.org/Team:Paris/23_July_2009 23] [http://2009.igem.org/Team:Paris/24_July_2009 24] [http://2009.igem.org/Team:Paris/25_July_2009 25] [http://2009.igem.org/Team:Paris/26_July_2009 26]
[http://2009.igem.org/Team:Paris/27_July_2009 27] [http://2009.igem.org/Team:Paris/28_July_2009 28] [http://2009.igem.org/Team:Paris/29_July_2009 29] [http://2009.igem.org/Team:Paris/30_July_2009 30] [http://2009.igem.org/Team:Paris/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/Team:Paris/1_August_2009 1] [http://2009.igem.org/Team:Paris/2_August_2009 2]
[http://2009.igem.org/Team:Paris/3_August_2009 3] [http://2009.igem.org/Team:Paris/4_August_2009 4] [http://2009.igem.org/Team:Paris/5_August_2009 5] [http://2009.igem.org/Team:Paris/6_August_2009 6] [http://2009.igem.org/Team:Paris/7_August_2009 7] [http://2009.igem.org/Team:Paris/8_August_2009 8] [http://2009.igem.org/Team:Paris/9_August_2009 9]
[http://2009.igem.org/Team:Paris/10_August_2009 10] [http://2009.igem.org/Team:Paris/11_August_2009 11] [http://2009.igem.org/Team:Paris/12_August_2009 12] [http://2009.igem.org/Team:Paris/13_August_2009 13] [http://2009.igem.org/Team:Paris/14_August_2009 14] [http://2009.igem.org/Team:Paris/15_August_2009 15] [http://2009.igem.org/Team:Paris/16_August_2009 16]
[http://2009.igem.org/Team:Paris/17_August_2009 17] [http://2009.igem.org/Team:Paris/18_August_2009 18] [http://2009.igem.org/Team:Paris/19_August_2009 19] [http://2009.igem.org/Team:Paris/20_August_2009 20] [http://2009.igem.org/Team:Paris/21_August_2009 21] [http://2009.igem.org/Team:Paris/22_August_2009 22] [http://2009.igem.org/Team:Paris/23_August_2009 23]
[http://2009.igem.org/Team:Paris/24_August_2009 24] [http://2009.igem.org/Team:Paris/25_August_2009 25] [http://2009.igem.org/Team:Paris/26_August_2009 26] [http://2009.igem.org/Team:Paris/27_August_2009 27] [http://2009.igem.org/Team:Paris/28_August_2009 28] [http://2009.igem.org/Team:Paris/29_August_2009 29] [http://2009.igem.org/Team:Paris/30_August_2009 30]
[http://2009.igem.org/Team:Paris/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Team:Paris/1_September_2009 1] [http://2009.igem.org/Team:Paris/2_September_2009 2] [http://2009.igem.org/Team:Paris/3_September_2009 3] [http://2009.igem.org/Team:Paris/4_September_2009 4] [http://2009.igem.org/Team:Paris/5_September_2009 5] [http://2009.igem.org/Team:Paris/6_September_2009 6]
[http://2009.igem.org/Team:Paris/7_September_2009 7] [http://2009.igem.org/Team:Paris/8_September_2009 8] [http://2009.igem.org/Team:Paris/9_September_2009 9] [http://2009.igem.org/Team:Paris/10_September_2009 10] [http://2009.igem.org/Team:Paris/11_September_2009 11] [http://2009.igem.org/Team:Paris/12_September_2009 12] [http://2009.igem.org/Team:Paris/13_September_2009 13]
[http://2009.igem.org/Team:Paris/14_September_2009 14] [http://2009.igem.org/Team:Paris/15_September_2009 15] [http://2009.igem.org/Team:Paris/16_September_2009 16] [http://2009.igem.org/Team:Paris/17_September_2009 17] [http://2009.igem.org/Team:Paris/18_September_2009 18] [http://2009.igem.org/Team:Paris/19_September_2009 19] [http://2009.igem.org/Team:Paris/20_September_2009 20]
[http://2009.igem.org/Team:Paris/21_September_2009 21] [http://2009.igem.org/Team:Paris/22_September_2009 22] [http://2009.igem.org/Team:Paris/23_September_2009 23] [http://2009.igem.org/Team:Paris/24_September_2009 24] [http://2009.igem.org/Team:Paris/25_September_2009 25] [http://2009.igem.org/Team:Paris/26_September_2009 26] [http://2009.igem.org/Team:Paris/27_September_2009 27]
[http://2009.igem.org/Team:Paris/28_September_2009 28] [http://2009.igem.org/Team:Paris/29_September_2009 29] [http://2009.igem.org/Team:Paris/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Team:Paris/1_October_2009 1] [http://2009.igem.org/Team:Paris/2_October_2009 2] [http://2009.igem.org/Team:Paris/3_October_2009 3] [http://2009.igem.org/Team:Paris/4_October_2009 4]
[http://2009.igem.org/Team:Paris/5_October_2009 5] [http://2009.igem.org/Team:Paris/6_October_2009 6] [http://2009.igem.org/Team:Paris/7_October_2009 7] [http://2009.igem.org/Team:Paris/8_October_2009 8] [http://2009.igem.org/Team:Paris/9_October_2009 9] [http://2009.igem.org/Team:Paris/10_October_2009 10] [http://2009.igem.org/Team:Paris/11_October_2009 11]
[http://2009.igem.org/Team:Paris/12_October_2009 12] [http://2009.igem.org/Team:Paris/13_October_2009 13] [http://2009.igem.org/Team:Paris/14_October_2009 14] [http://2009.igem.org/Team:Paris/15_October_2009 15] [http://2009.igem.org/Team:Paris/16_October_2009 16] [http://2009.igem.org/Team:Paris/17_October_2009 17] [http://2009.igem.org/Team:Paris/18_October_2009 18]
[http://2009.igem.org/Team:Paris/19_October_2009 19] [http://2009.igem.org/Team:Paris/20_October_2009 20] [http://2009.igem.org/Team:Paris/21_October_2009 21] [http://2009.igem.org/Team:Paris/22_October_2009 22] [http://2009.igem.org/Team:Paris/23_October_2009 23] [http://2009.igem.org/Team:Paris/24_October_2009 24] [http://2009.igem.org/Team:Paris/25_October_2009 25]
[http://2009.igem.org/Team:Paris/26_October_2009 26] [http://2009.igem.org/Team:Paris/27_October_2009 27] [http://2009.igem.org/Team:Paris/28_October_2009 28] [http://2009.igem.org/Team:Paris/29_October_2009 29] [http://2009.igem.org/Team:Paris/30_October_2009 30] [http://2009.igem.org/Team:Paris/31_October_2009 31]

← Yesterday - Tomorrow →

August 12th


Lab work

Ligation

D15 (2xterminator E/S) and D16 (pLac E/X) with vector psb2k3

DO : D15=1,19µg/mL D16=1,75µg/mL

vecteur insert H20 Buffer 10x T4 ligase T4 ligase Total
L5 2xterm:pSB2K3(with pLac) 1µl 3µl 13µl 2µl 1µl 20µl
control - o 1µl o 15µl 2µl 1µl 20µl


1h Home temperature

Transformation

classic protocol with DH5a

Purification

  • Purification of A10(ClyA) (PCR at 55° during 30sec) (using 1% agarose during 25 min)
  • A10=A10 987pb
ClyA pcr2.JPG
Cly1 pcr3.JPG
ClyA coupé.JPG
  • A10 purificated
ClyA purif.JPG
  • Purification of A10 work

PCR Gel verification

1% aga gel after PCR under different conditions for A18 (OmpA-Linker from Varsaw). Expected weight : 525bp.

Gel 090812Sylvain.JPG

Obviously, something has gone wrong. Next step will be different temperature gradients testing for PCR and/or new matrix.

Transformation

Classic protocol with DH5a, and pSB2K3 from iGEM DNA plates (1/7C). ON culture on LB + Kan.

To be checked tomorrow !

ON culture for Minipreps tomorrow

pSB1A3, pLAC, RBSTetR, OmpA-L, Three ligation clones, ON culture in LB + Abiotics. 37°C with stiring.

ON PCR

A20 : 49, 51 and 53°C PCR with the plasmid for OmpA-L. Overnigth. 15s elongation time.

All Manip

Comming soon

To do list

Matricule TODO
Luc
Romain
Charlotte
Stoff
Chris
Lisa
Caroline
Souf
Vicard
Pierre
Sylvain pSB2K3 transformation ; PCR gels and purifs or new culture for minipreps
Guillaume


← Yesterday - Tomorrow →

Retrieved from "http://2009.igem.org/Team:Paris/12_August_2009"