Team:TUDelft/18 August 2009

From 2009.igem.org

(Difference between revisions)
(→18 August 2009)
Line 4: Line 4:
='''18 August 2009'''=
='''18 August 2009'''=
 +
 +
===Calin===
 +
 +
Yesterdays 20 well 1% gel:
 +
 +
 +
{| border="1" align="center"
 +
| Well || Part || Expected Plasmid Size || Status
 +
|- align="center"
 +
| 1 || || ||
 +
|- align="center"
 +
| 2 ||  || ||
 +
|- align="center"
 +
| 3 ||  || ||
 +
|- align="center"
 +
| 4 ||  || ||
 +
|- align="center"
 +
| 5 ||  || ||
 +
|- align="center"
 +
| 6 ||  [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 7 || Colony PCR 1A - R751&#916;trbK || 1616 ||
 +
|- align="center"
 +
| 8 || Colony PCR 1A - R751&#916;trbK || 1616 ||
 +
|- align="center"
 +
| 9 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  ||
 +
|- align="center"
 +
| 10 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||
 +
|- align="center"
 +
| 11 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||
 +
|- align="center"
 +
| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
 +
|}
 +
 +
Yesterdays 26 well 2% gel:
 +
 +
 +
{| border="1" align="center"
 +
| Well || Part || Expected Plasmid Size || Status
 +
|- align="center"
 +
| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 2 ||  || ||
 +
|- align="center"
 +
| 3 ||  || ||
 +
|- align="center"
 +
| 4 ||  || ||
 +
|- align="center"
 +
| 5 ||  || ||
 +
|- align="center"
 +
| 6 ||  || ||
 +
|- align="center"
 +
| 7 ||  || ||
 +
|- align="center"
 +
| 8 ||  || ||
 +
|- align="center"
 +
| 9 ||  ||  ||
 +
|- align="center"
 +
| 10 ||  || ||
 +
|- align="center"
 +
| 11 ||  || ||
 +
|- align="center"
 +
| 12 ||  || ||
 +
|- align="center"
 +
| 13 ||  || ||
 +
|- align="center"
 +
| 14 ||  || ||
 +
|- align="center"
 +
| 15 ||  || ||
 +
|- align="center"
 +
| 16 ||  || ||
 +
|- align="center"
 +
| 17 ||  || ||
 +
|- align="center"
 +
| 18 ||  || ||
 +
|- align="center"
 +
| 19 ||  || ||
 +
|- align="center"
 +
| 20 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 21 ||  || ||
 +
|- align="center"
 +
| 22 || Colony PCR 1A - R751&#916;trbK || 1616 ||
 +
|- align="center"
 +
| 23 || Colony PCR 1A - R751&#916;trbK || 1616 ||
 +
|- align="center"
 +
| 24 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  ||
 +
|- align="center"
 +
| 25 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||
 +
|- align="center"
 +
| 26 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||
 +
|- align="center"
 +
|}
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 17:52, 17 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

18 August 2009

Calin

Yesterdays 20 well 1% gel:


Well Part Expected Plasmid Size Status
1
2
3
4
5
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
7 Colony PCR 1A - R751ΔtrbK 1616
8 Colony PCR 1A - R751ΔtrbK 1616
9 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
10 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
11 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔

Yesterdays 26 well 2% gel:


Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
21
22 Colony PCR 1A - R751ΔtrbK 1616
23 Colony PCR 1A - R751ΔtrbK 1616
24 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
25 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
26 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398

Retrieved from "http://2009.igem.org/Team:TUDelft/18_August_2009"