Team:TUDelft/18 August 2009

From 2009.igem.org

(Difference between revisions)
(→Calin)
(→Calin)
Line 8: Line 8:
Yesterdays 20 well 1% gel:
Yesterdays 20 well 1% gel:
 +
 +
[[Image:Gel17082009-20well-1pc-sd-c.png|600px]]
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| 8 || Colony PCR 1A - R751ΔtrbK || 1616 ||  
| 8 || Colony PCR 1A - R751ΔtrbK || 1616 ||  
|- align="center"
|- align="center"
-
| 9 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  ||  
+
| 9 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  || <font color=limegreen>&#10004;</font>
|- align="center"
|- align="center"
| 10 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||  
| 10 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||  
|- align="center"
|- align="center"
-
| 11 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||  
+
| 11 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 || <font color=limegreen>&#10004;</font>
|- align="center"
|- align="center"
| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  || <font color=limegreen>&#10004;</font>
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Yesterdays 26 well 2% gel:
Yesterdays 26 well 2% gel:
 +
[[Image:Gel17082009-26well-2pc-dela.png|600px]]
{| border="1" align="center"
{| border="1" align="center"
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| 23 || Colony PCR 1A - R751&#916;trbK || 1616 ||  
| 23 || Colony PCR 1A - R751&#916;trbK || 1616 ||  
|- align="center"
|- align="center"
-
| 24 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  ||  
+
| 24 || Colony PCR R751 + pKD46 + trbK_KO_mod_S || 348  || <font color=limegreen>&#10004;</font>
|- align="center"
|- align="center"
| 25 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||  
| 25 || Colony PCR R751&#916;oriTR -L-ara +PCR control || ? ||  
|- align="center"
|- align="center"
-
| 26 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||  
+
| 26 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 || <font color=limegreen>&#10004;</font>
|- align="center"
|- align="center"
|}
|}

Revision as of 07:29, 18 August 2009

Lab Notebook

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18 August 2009

Calin

Yesterdays 20 well 1% gel:

Gel17082009-20well-1pc-sd-c.png


Well Part Expected Plasmid Size Status
1
2
3
4
5
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
7 Colony PCR 1A - R751ΔtrbK 1616
8 Colony PCR 1A - R751ΔtrbK 1616
9 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348 ✔
10 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
11 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398 ✔
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔

Yesterdays 26 well 2% gel:

Gel17082009-26well-2pc-dela.png

Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
20
21
22 Colony PCR 1A - R751ΔtrbK 1616
23 Colony PCR 1A - R751ΔtrbK 1616
24 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348 ✔
25 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
26 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398 ✔


For the trbK conjugation test 24 plates were made:


Set 1
Plate ID Antibiotics Dilution
D1tr TRI 100
D2tr TRI 10-1
D3tr TRI 10-2
D4tr TRI 10-3
D5tr TRI 10-4
T1tr TRI + CAM 100
T2tr TRI + CAM 10-1
T3tr TRI + CAM 10-2
T4tr TRI + CAM 10-3
T5tr TRI + CAM 10-4
R1tr CAM 100
R2tr CAM 10-1



Set 2
Plate ID Antibiotics Dilution
D6tr TRI 100
D7tr TRI 10-1
D8tr TRI 10-2
D9tr TRI 10-3
D10tr TRI 10-4
T6tr TRI + CAM 100
T7tr TRI + CAM 10-1
T8tr TRI + CAM 10-2
T9tr TRI + CAM 10-3
T10tr TRI + CAM 10-4
R3tr CAM 100
R4tr CAM 10-1

For the oriTR conjugation test 51 plates were made:

Set 1
Plate ID Antibiotics Dilution
D1ori TRI + CAM 100
D2ori TRI + CAM 10-1
D3ori TRI + CAM 10-2
D4ori TRI + CAM 10-3
D5ori TRI + CAM 10-4
Ts1ori AMP + CAM 100
Ts2ori AMP + CAM 10-1
Ts3ori AMP + CAM 10-2
Ts4ori AMP + CAM 10-3
Ts5ori AMP + CAM 10-4
TL1ori TRI + AMP 100
TL2ori TRI + AMP 10-1
TL3ori TRI + AMP 10-2
TL4ori TRI + AMP 10-3
TL5ori TRI + AMP 10-4
R1ori AMP 100
R2ori AMP 10-1



Set 2
Plate ID Antibiotics Dilution
D6ori TRI + CAM 100
D7ori TRI + CAM 10-1
D8ori TRI + CAM 10-2
D9ori TRI + CAM 10-3
D10ori TRI + CAM 10-4
Ts6ori AMP + CAM 100
Ts7ori AMP + CAM 10-1
Ts8ori AMP + CAM 10-2
Ts9ori AMP + CAM 10-3
Ts10ori AMP + CAM 10-4
TL6ori TRI + AMP 100
TL7ori TRI + AMP 10-1
TL8ori TRI + AMP 10-2
TL9ori TRI + AMP 10-3
TL10ori TRI + AMP 10-4
R3ori AMP 100
R4ori AMP 10-1




Set 3
Plate ID Antibiotics Dilution
D11ori TRI + CAM 100
D12ori TRI + CAM 10-1
D13ori TRI + CAM 10-2
D14ori TRI + CAM 10-3
D15ori TRI + CAM 10-4
Ts11ori AMP + CAM 100
Ts12ori AMP + CAM 10-1
Ts13ori AMP + CAM 10-2
Ts14ori AMP + CAM 10-3
Ts15ori AMP + CAM 10-4
TL11ori TRI + AMP 100
TL12ori TRI + AMP 10-1
TL13ori TRI + AMP 10-2
TL14ori TRI + AMP 10-3
TL15ori TRI + AMP 10-4
R5ori AMP 100
R6ori AMP 10-1

Retrieved from "http://2009.igem.org/Team:TUDelft/18_August_2009"