Team:Paris/Freezer Primers

From 2009.igem.org

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<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Primers#bottom | Primers]]
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[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -  
[[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -  
[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -  
[[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -  
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[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]]
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[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] - [[Team:Paris/constructions#Freezer | Construction in progress]]
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! colspan="6" style="background: #c5c5c5;" | <span/ id="O32"> [O31] clyA_RBS_Fw
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! colspan="6" style="background: #c5c5c5;" | <span/ id="O32"> [O32] clyA fusion_polyG_Fw
|- style="background: #cbcee0;"
|- style="background: #cbcee0;"
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
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|width=700px| amplify clyA with RBS in the primer (std 10)
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|width=700px| clyA fusion Fw (with 5-Gly linker)
|- style="background: #cbcee0;"
|- style="background: #cbcee0;"
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
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|TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC
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|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGCGGCGGCGGCACTGAAATCGTTGCAGATAAAAC
|- style="background: #cbcee0;"
|- style="background: #cbcee0;"
|style="background:#0d3e99; color:white; text-align: right;"|Designer
|style="background:#0d3e99; color:white; text-align: right;"|Designer
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|- style="background: #cbcee0;"
|style="background: #0d3e99; color:white;  text-align: right;" | Status
|style="background: #0d3e99; color:white;  text-align: right;" | Status
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| style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
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| style="font-weight:bold;" |Ordered: 07/27 ; Received: no ; Used in wet lab: no
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([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
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Latest revision as of 13:42, 28 August 2009

iGEM > Paris > Freezer > Primers

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress

Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Primers

[O1] Rev P1
Description tolR
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O2] Fwd P2 (ERROR : Do not use)
Description tolR
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O3] Rev P3
Description tolA
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O4] Rev P4 (ERROR: Not designed for N-term fusion)
Description tolA
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O5] Rev P5
Description tatABC
Sequence (5' > 3') TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[06] Fwd P6 (ERROR: without RBS)
Description tatABC
Sequence (5' > 3') TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O7] Rev P7 (ERROR: not the good standard for fusion)
Description clyA fusion
Sequence (5' > 3') TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O8] Fwd P8 (ERROR: No poly-Gly linker)
Description clyA fusion
Sequence (5' > 3') TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA
Designer D. Bikard
Status Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)


[O9] clyA_Rw
Description clyA (for N term fusion)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: no


[O10] clyA_fusion_polyG_Rw
Description clyA fusion (with 5-Gly linker)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O11] ompA_signal_Fw
Description ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O12] ompA_signal_fusion_Rw
Description ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O13] tolR_II_Fusion_Fw
Description For fusion of tolR domain II (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)

[O14] tatABC_RBS_Fw
Description Amplification from tatA - contains RBS
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O15] TE3_fusion_Fw
Description Fusion of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC
Designer C.Loy
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O16] TE3_Rw
Description Amplification of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG
Designer C.Loy
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O17] Tg3p_Rw
Description Amplification of N-term domain of M13's g3p
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC
Designer C. Loy
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O18] Tg3p_fusion_Fw
Description Fusion of N-term domain of M13's g3p (standard 23)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG
Designer C.Loy
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O19] g3p_F
Description g3p
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG
Designer G. Beauclair
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O20] g3p_R
Description g3p
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG
Designer G. Beauclair
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no
[O21] Ompa_linker_F
Description For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG
Designer G. Beauclair
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O22] Ompa_linker_R
Description For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC
Designer G. Beauclair
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O23] OmpAsignal-TE3_seq_Fw
Description For sequencing of part OmpAsignal-TE3
Sequence (5' > 3') CACAAATAGCGAAAGATGAC
Designer C.Loy
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O24] OmpAsignal-TE3_seq_Rw
Description For sequencing of part OmpAsignal-TE3
Sequence (5' > 3') ATCATCTGCGGGTAATGATG
Designer C.Loy
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O25] VAMP2_Seq_Fw
Description For sequencing of VAMP2 (snare)
Sequence (5' > 3') ACTCACTATAGGGAGACCACA
Designer S.Boumahdi
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O26] VAMP2_Seq_Rv
Description For sequencing of VAMP2 (snare)
Sequence (5' > 3') GGGCTTTGTTAGCAGCCGGAT
Designer S.Boumahdi
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O27] ClyA_seq_Fw
Description For sequencing of clyA after amplification
Sequence (5' > 3') GCAGCTATTTCCAGTCACAG
Designer V.Du
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O28] ClyA_seq_Rw
Description For sequencing of clyA after amplification
Sequence (5' > 3') CGCCCGCAGCAATAGAATAG
Designer V.Du
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O29] PFecA_Fw
Description For signal transduction
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC
Designer R.Bodinier
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O30] PFecA_Rv
Description For signal transduction
Sequence (5' > 3') GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT
Designer R.Bodinier
Status Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no


[O31] clyA_RBS_Fw
Description amplify clyA with RBS in the primer (std 10)
Sequence (5' > 3') TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC
Designer G. Cambray
Status Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes

(08/05)


[O32] clyA fusion_polyG_Fw
Description clyA fusion Fw (with 5-Gly linker)
Sequence (5' > 3') GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGCGGCGGCGGCACTGAAATCGTTGCAGATAAAAC
Designer G. Cambray
Status Ordered: 07/27 ; Received: no ; Used in wet lab: no

( )

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