Team:UNICAMP-Brazil/Notebooks/September 29

From 2009.igem.org

(Difference between revisions)
(→Dephosphorylation - CIAP test)
Line 7: Line 7:
====Dephosphorylation - CIAP test====
====Dephosphorylation - CIAP test====
-
- Only a few colonies were found this morning =(. Unfortunately the number of colonies wasn´t enough to compare and decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.
+
*<p style=”text-align:justify;”>Only a few colonies were found this morning =(. Unfortunately the number of colonies wasn´t enough to compare and decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.</p>
====New biobricks====
====New biobricks====
-
- Today we performed 5' dephosphorylation of the biofusion vector with SAP (Protocol 9). We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).
+
*<p style=”text-align:justify;”>Today we performed 5' dephosphorylation of the biofusion vector with SAP [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation Protocol 9]. We did the ligation reaction ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/T4_DNA_Ligase Protocol 11]) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).</p>
-
- We transformed the thermocompetent E. coli (protocol 3) with the ligations and plated in LB+Amp media.
+
*<p style=”text-align:justify;”>We transformed the thermocompetent E. coli ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Electroporation Protocol 3]) with the ligations and plated in LB+Amp media.</p>

Revision as of 21:17, 3 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

Dephosphorylation - CIAP test

  • Only a few colonies were found this morning =(. Unfortunately the number of colonies wasn´t enough to compare and decide between CIAP and SAP. So we decided to use SAP to dephosphorylate our biofusion vector, considering that the protocol is much easier.

New biobricks

  • Today we performed 5' dephosphorylation of the biofusion vector with SAP Protocol 9. We did the ligation reaction (Protocol 11) with the 5' dephosphorylated vector and the three digested (XbaI and SpeI) new parts (pJen1, pDLD and Lysozyme).

  • We transformed the thermocompetent E. coli (Protocol 3) with the ligations and plated in LB+Amp media.


Raíssa and Taís





Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/September_29"