Team:PKU Beijing/Project/AND Gate 1/Design
From 2009.igem.org
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[[Image:PKU_Generator_Promoter_Reverse.png|width='10']] | [[Image:PKU_Generator_Promoter_Reverse.png|width='10']] | ||
[[Image:PKU_Generater_Promoter_Forward.png]] | [[Image:PKU_Generater_Promoter_Forward.png]] | ||
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fig3. Two ways to arrange repressor(activator) generater and the corresponding promoter | fig3. Two ways to arrange repressor(activator) generater and the corresponding promoter | ||
+ | 1. The constitutive promoter is cloned back to back with the inducible promoter, thus transcription of the activator or the repressor is opposite of the inducible promoter. 2. The generator transcripts in the same direction of the inducible promoter, the constitutive promoter is prevented to interfere with the inducible promoter by the terminator. However, any terminator is leaky, the first way is the better way to prevent interference. | ||
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+ | This construct combines repressors (activators) together with its promoter. It is actually a quite useful part even outside of the AND gate Module. We call it an environmental small molecule sensor. It provides a sensor for environmental signals and a interface to intracellular circuits. Actually, a similar construct(repressor protein only) without the constitutive promoter is called an inverter, but we think it is more proper to be called a sensor when the promoter upstream of the repressor is a constitutive promoter. Its signaling molecule like aTc, IPTG, arabinose, HomoSerineLactone and salicylate diffuse into the cell and take function. It is like the some of the hydrophobic hormones in high organisms. | ||
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+ | The promoters we chose at first is the LuxR-LuxP construct induced by Homoserinelactone, the tet operon induced by aTc, the Lac operon induced by IPTG, and the Arabinose inducible promoter PBad. So we designed the following sensors: | ||
+ | [[Image:PKU_aTc_Sensor]] | ||
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{{PKU_Beijing/Foot}} | {{PKU_Beijing/Foot}} |
Revision as of 11:21, 10 October 2009
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