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Team:Paris/Production testing delay
From 2009.igem.org
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[[Team:Paris/Production_testing_delay#part1 | Part1 : testing delay phase 1]] | [[Team:Paris/Production_testing_delay#part1 | Part1 : testing delay phase 1]] | ||
| - | [[Team:Paris/Production_testing_delay#functional testing of the part 1 | functional testing of the part 1]] | + | [[Team:Paris/Production_testing_delay#functional testing of the part 1 | Part1 : functional testing of the part 1]] |
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[[Team:Paris/Production_testing_delay#part2 | Part2 : testing delay phase 2]] | [[Team:Paris/Production_testing_delay#part2 | Part2 : testing delay phase 2]] | ||
| - | [[Team:Paris/Production_testing_delay#functional testing of the part 2 | functional testing of the part 2]] | + | [[Team:Paris/Production_testing_delay#functional testing of the part 2 | Part2 : functional testing of the part 2]] |
Latest revision as of 14:43, 18 October 2009
iGEM > Paris > Production > Testing > delay
Delay
We separate the construction on two plasmids :
Part1 : functional testing of the part 1
Part2 : functional testing of the part 2
part1
Time required : A lOT !!!!!
Experiments ran :
| column 1 | column 2 | column 3 | column 4
|
| PCR :
TatABCE matrix : | PCR : | PCR : | PCR : |
| Verification on gel :
ok | Verification on gel :
- | Verification on gel :
- | Verification on gel :
- |
| Purification on gel :
ok | Purification on gel :
- | Purification on gel :
- | Purification on gel :
-
|
| digestion:
TatABCE | digestion: | digestion: | digestion: |
| verification digestion:
ok | verification digestion:
- | verification digestion:
- | verification digestion:
-
|
| ligation:
TatABCE | ligation: | ligation: | ligation: |
| PCR colo :
ok | PCR colo :
- | PCR colo :
- | PCR colo :
- |
| miniprep:
STOPPED | miniprep:
- | miniprep:
- | miniprep:
- |
| sequencing :
- | sequencing :
- | sequencing :
- | sequencing :
- |
functional testing of the part 1
PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :
1% arabinose
1% glucose
LB pure
part2
Time required : A lOT !!!!!
Experiments ran :
| column 1 | column 2 | column 3 | column 4
|
| PCR :
TatABCE matrix : | PCR : | PCR : | PCR : |
| Verification on gel :
ok | Verification on gel :
- | Verification on gel :
- | Verification on gel :
- |
| Purification on gel :
ok | Purification on gel :
- | Purification on gel :
- | Purification on gel :
-
|
| digestion:
TatABCE | digestion: | digestion: | digestion: |
| verification digestion:
ok | verification digestion:
- | verification digestion:
- | verification digestion:
-
|
| ligation:
TatABCE | ligation: | ligation: | ligation: |
| PCR colo :
ok | PCR colo :
- | PCR colo :
- | PCR colo :
- |
| miniprep:
STOPPED | miniprep:
- | miniprep:
- | miniprep:
- |
| sequencing :
- | sequencing :
- | sequencing :
- | sequencing :
- |
functional testing of the part 2
Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :
0,1% IPTG .
1% glucose.
LB pure.
