Team:TUDelft/21 August 2009

From 2009.igem.org

(Difference between revisions)
(21 August 2009)
(Sriram)
 
(One intermediate revision not shown)
Line 5: Line 5:
='''21 August 2009'''=
='''21 August 2009'''=
===Sriram===
===Sriram===
-
Today i did miniprep of the biobricks needed for ribo-regulator
+
Today i did miniprep of the biobricks needed for ribo-regulator.
 +
 
===Saeed===
===Saeed===
!Fr1n downsteam was cut out agarose gel of 19-08-2009 and extracted by gel extraction kit. After extraction DNA concentration was 3.7 ng/microL. !C was digested by SpeI and PstI and !Fr1n downsteam is ligated into this. This construct is called !Z. After ligation !Z was transformed into electrocompetent cells and grown outside the incubator during weekend.
!Fr1n downsteam was cut out agarose gel of 19-08-2009 and extracted by gel extraction kit. After extraction DNA concentration was 3.7 ng/microL. !C was digested by SpeI and PstI and !Fr1n downsteam is ligated into this. This construct is called !Z. After ligation !Z was transformed into electrocompetent cells and grown outside the incubator during weekend.

Latest revision as of 01:38, 22 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

21 August 2009

Sriram

Today i did miniprep of the biobricks needed for ribo-regulator.

Saeed

!Fr1n downsteam was cut out agarose gel of 19-08-2009 and extracted by gel extraction kit. After extraction DNA concentration was 3.7 ng/microL. !C was digested by SpeI and PstI and !Fr1n downsteam is ligated into this. This construct is called !Z. After ligation !Z was transformed into electrocompetent cells and grown outside the incubator during weekend.

Tim Weenink

Tim210809.png

Calin

Did colony PCR with Taq on 4 R751_oriTR_KO colonies and 2 R751_trbK_KO colonies.

Mix shotgun sequencing mixes:
CA 1223 bp: A_VF, A_VR
CB 1162 bp: B_VF, B_VR
CC 311 bp: C_VF, C_VR
CE 231 bp: E_VF, E_VR