Team:TUDelft/3 July 2009

From 2009.igem.org

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(New page: {{Template:TUDelftiGEM2009}} ='''Home'''= This is the first template of the TU Delft 2009 iGEM team wiki! On this page information about the project and its progress can be found. <br><br...)
 
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='''Home'''=
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This is the first template of the TU Delft 2009 iGEM team wiki! On this page information about the project and its progress can be found.
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<br><br>
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<!-- |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.-->
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==Welcome==
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Welcome to the Wiki of the Technische Universiteit Delft team for iGEM 2009. '''The site is still under construction''', but as soon as it is finished, we hope to keep with the standards of the TU Delft team last year.
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|[[Image:Logo_TUDelft.png|50px|center|frame]]
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<!--- ''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''--->
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<br><br>
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== The Team ==
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This is the second year the TUDelft participates in the iGEM competition.
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The group consists of 7 students, 4 instructors and a lot of advisors from TUDelft willing to help out and think with us when it is necessary. An overview of the people involved and our competences can be found on the [[Team:TUDelft/Team|team]] page.
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With our 7 participants and 4 instructors, we are enthusiastic to start working on our project: Self degrading plasmids.
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|align="center"|[[Team:TUDelft | TUDelft iGEM team of 2009]]
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='''3rd July'''=
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=='''Conjugation'''==
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Today, we made an unbelievable discovery: we found out that the conjugation between two cells with the same plasmids has been proposed before by the [https://2007.igem.org/Peking_Hop-Count Peking team from iGEM 2007]. In conjugation, the Tra family proteins are present on the F plasmid and are responsible for establishing the connection between the conjugating cells. The Peking team took traI out of the F plasmid, and implemented it into a miniF plasmid to allow only cells who contain the miniF plasmid would be able to conjugate with other cells. The problem that Peking had with using traI was that it had several restriction sites that were not allowed by the iGEM standards. Nevertheless, we looked today at a small part from the traI that had crucial genes for conjugation and did not contain illegal segments. We are still not sure whether this gene will be removed properly from the F plasmid and function well within the miniF, so we are still trying to consider some alternative tra genes in the F plasmid that are important to conjugation.
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Latest revision as of 14:26, 25 July 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

3rd July


Conjugation

Today, we made an unbelievable discovery: we found out that the conjugation between two cells with the same plasmids has been proposed before by the Peking team from iGEM 2007. In conjugation, the Tra family proteins are present on the F plasmid and are responsible for establishing the connection between the conjugating cells. The Peking team took traI out of the F plasmid, and implemented it into a miniF plasmid to allow only cells who contain the miniF plasmid would be able to conjugate with other cells. The problem that Peking had with using traI was that it had several restriction sites that were not allowed by the iGEM standards. Nevertheless, we looked today at a small part from the traI that had crucial genes for conjugation and did not contain illegal segments. We are still not sure whether this gene will be removed properly from the F plasmid and function well within the miniF, so we are still trying to consider some alternative tra genes in the F plasmid that are important to conjugation.

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