Team:TUDelft/29 July 2009

From 2009.igem.org

(Difference between revisions)
(New page: {{Template:TUDelftiGEM2009}} ='''29 July 2009'''= {{Template:TUDelftiGEM2009_end}})
(→Calin)
 
(16 intermediate revisions not shown)
Line 1: Line 1:
{{Template:TUDelftiGEM2009}}
{{Template:TUDelftiGEM2009}}
 +
 +
{{Template:TUDelftiGEM2009_LabNotebook}}
='''29 July 2009'''=
='''29 July 2009'''=
 +
 +
===Calin===
 +
 +
Checked concentrations from yesterday's miniprep.
 +
 +
{| border="1" align="center"
 +
| Part || Concentration (ng/uL)
 +
|- align="center"
 +
| pSB1AK3 I|| 92
 +
|- align="center"
 +
| pSB1AK3 II || 77
 +
|- align="center"
 +
| lambda_p GFP I || 119
 +
|- align="center"
 +
| lambda_p GFP II || 108
 +
|- align="center"
 +
| pLacI || 56.7
 +
|- align="center"
 +
| RBS || 95.2
 +
|}
 +
 +
Todays gel:
 +
 +
[[Image:Gel29072009.jpg|thumb|center|450px]]
 +
 +
 +
 +
{| border="1" align="center"
 +
| Well || Part || Expected Plasmid Size || Status
 +
|- align="center"
 +
| 1 || BBa_K142205 PCR || ||
 +
|- align="center"
 +
| 2 || BBa_K142202 PCR ||  ||
 +
|- align="center"
 +
| 3 || BBa_B0015 PCR ||  ||
 +
|- align="center"
 +
| 4 || BBa_R0040 PCR ||  ||
 +
|- align="center"
 +
| 5 || BBa_K145201 PCR ||  ||
 +
|- align="center"
 +
| 6 || *I3 uncut ||  ||
 +
|- align="center"
 +
| 7 || *I3 BglI cut ||  ||
 +
|- align="center"
 +
| 8 || *I3 BglI+I-SceI cut ||  ||
 +
|- align="center"
 +
| 9 || *I6 uncut ||  ||
 +
|- align="center"
 +
| 10 || *I6 BglI cut ||  ||
 +
|- align="center"
 +
| 11 || *I7 BglI+I-SceI cut ||  ||
 +
|- align="center"
 +
| 12 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||
 +
|- align="center"
 +
| 13 || pSB1AK3 I || 3864 || <font color=red>&#10006;</font>
 +
|- align="center"
 +
| 14 || pSB1AK3 II || 3864 || <font color=red>&#10006;</font>
 +
|- align="center"
 +
| 15 || &lambda;p-GFP I || 3011 || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 16 || &lambda;p-GFP II || 3011 || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 17 || pLacI || 2279 || <font color=limegreen>&#10004;</font>
 +
|- align="center"
 +
| 18 || rbs || 2091 ||  <font color=limegreen>&#10004;</font>
 +
|}
 +
 +
===Sriram===
 +
We planned to make electro competent cells today but we got the required OD of 0.5 to 1 a bit late. We hoped to make cells if the meeting finishes quicker, but sadly it took longer than expected hence we had to waste the culture that was grown.
 +
Laura gave a new [https://2009.igem.org/Team:TUDelft/Protocols#Prepering_chemically_competant_cells_-_TMF_Buffer  protocol] for preparing chemically competent cells and also 6 ml of buffer required. We decided to prepare the cells tomorrow, hence I again made some 3 culture tubes of top10 cells.
 +
 +
===Orr===
 +
Got the source code in R for the modelling of conjugation, and already looked at how it works.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Latest revision as of 10:30, 12 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

29 July 2009

Calin

Checked concentrations from yesterday's miniprep.

Part Concentration (ng/uL)
pSB1AK3 I 92
pSB1AK3 II 77
lambda_p GFP I 119
lambda_p GFP II 108
pLacI 56.7
RBS 95.2

Todays gel:

Gel29072009.jpg


Well Part Expected Plasmid Size Status
1 BBa_K142205 PCR
2 BBa_K142202 PCR
3 BBa_B0015 PCR
4 BBa_R0040 PCR
5 BBa_K145201 PCR
6 *I3 uncut
7 *I3 BglI cut
8 *I3 BglI+I-SceI cut
9 *I6 uncut
10 *I6 BglI cut
11 *I7 BglI+I-SceI cut
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
13 pSB1AK3 I 3864 ✖
14 pSB1AK3 II 3864 ✖
15 λp-GFP I 3011 ✔
16 λp-GFP II 3011 ✔
17 pLacI 2279 ✔
18 rbs 2091 ✔

Sriram

We planned to make electro competent cells today but we got the required OD of 0.5 to 1 a bit late. We hoped to make cells if the meeting finishes quicker, but sadly it took longer than expected hence we had to waste the culture that was grown. Laura gave a new protocol for preparing chemically competent cells and also 6 ml of buffer required. We decided to prepare the cells tomorrow, hence I again made some 3 culture tubes of top10 cells.

Orr

Got the source code in R for the modelling of conjugation, and already looked at how it works.

Retrieved from "http://2009.igem.org/Team:TUDelft/29_July_2009"