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Team:Paris/23 July 2009
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==NoteBook== | ==NoteBook== | ||
| - | { | + | {{Paris2009_Calendar}} |
| - | + | {{Paris2009_Calendar_Link|22_July_2009|24_July_2009}} | |
| - | + | <center> '''July 23th''' </center> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
<html> | <html> | ||
| Line 19: | Line 15: | ||
</html> | </html> | ||
| + | ===Brain work=== | ||
| - | = | + | *<u>Modelling:</u> |
| + | **Proteic binding force estimation for Tol/Pal system: | ||
| + | ***use a simple estimation based on the existing values.(E_Prot=10-4O kJ/Mol*N_Prot) | ||
| + | ***use a simple estimation based on the existing values of the OmpA familly. | ||
| + | ***Docking values given by our own work on TolA/Pal.(aborted) | ||
| + | **Osmotic pressure estimation | ||
| + | ***A mean on the wall cycle of the bacteria: 75 lb (bionumber estimation) | ||
| + | ***Osmolarity of the periplasm:~300 mOsm | ||
| + | **Total model: | ||
| + | ***Direct and simple shemtaics of the Model was designed: complet scheme begining of the choices for numerical values. | ||
| - | * | + | Sec-Tat Pathway: |
| + | *reading of 2 articles on tranlocation cinetics via Sec & Tat pathway | ||
| + | *the translocation rate of amino acids per time unit is constant on the Sec pathway | ||
| + | Ideas : | ||
| + | *Use a model of active diffusion across membrane | ||
| + | *Think about an evenemential simulation | ||
<html> | <html> | ||
| Line 32: | Line 43: | ||
</html> | </html> | ||
| - | ==Lab work== | + | ===Lab work=== |
| - | + | *DH5 alpha competent bacteria by [[Team:Paris/Protocols_Culture#CaCl2a | CaCl<sub>2</sub> protocol]] | |
| - | + | ||
| - | + | ||
<html> | <html> | ||
| Line 44: | Line 53: | ||
</html> | </html> | ||
| + | ===To do list=== | ||
| + | |||
| + | Find the constrcution that are necessary for the fec system that Braun can't provide us with. | ||
| + | |||
| + | |||
| + | Modelling | ||
| + | *Get data on osmotic pressure. | ||
| + | *Find information on active diffusion across membranes. | ||
| + | *Find kinetics datas for complexation of tol-pal interaction as well as the translocation via tat and Sec export system. | ||
| + | *Get a better understanding of PMF. | ||
| + | |||
| + | |||
| + | *Test genomic DNA purification of S2 | ||
| + | *More competent bacteria | ||
| - | |||
| - | + | {{Paris2009_Calendar_Link|22_July_2009|24_July_2009}} | |
Latest revision as of 13:11, 10 August 2009
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NoteBook
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Brain work
- Modelling:
- Proteic binding force estimation for Tol/Pal system:
- use a simple estimation based on the existing values.(E_Prot=10-4O kJ/Mol*N_Prot)
- use a simple estimation based on the existing values of the OmpA familly.
- Docking values given by our own work on TolA/Pal.(aborted)
- Osmotic pressure estimation
- A mean on the wall cycle of the bacteria: 75 lb (bionumber estimation)
- Osmolarity of the periplasm:~300 mOsm
- Total model:
- Direct and simple shemtaics of the Model was designed: complet scheme begining of the choices for numerical values.
- Proteic binding force estimation for Tol/Pal system:
Sec-Tat Pathway:
- reading of 2 articles on tranlocation cinetics via Sec & Tat pathway
- the translocation rate of amino acids per time unit is constant on the Sec pathway
Ideas :
- Use a model of active diffusion across membrane
- Think about an evenemential simulation
To do list
Find the constrcution that are necessary for the fec system that Braun can't provide us with.
Modelling
- Get data on osmotic pressure.
- Find information on active diffusion across membranes.
- Find kinetics datas for complexation of tol-pal interaction as well as the translocation via tat and Sec export system.
- Get a better understanding of PMF.
- Test genomic DNA purification of S2
- More competent bacteria
