Team:Paris/Freezer Plasmids

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Latest revision as of 15:27, 19 October 2009

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Plasmids

Color code for sticker
Vector	Insert	PCR product	Plasmid	Strains	Kanamycin	Ampicilin	Chloranphenicol

Plasmids: MiniPrep Products
Number	Name	Description	Products in use	Date
P1	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	General plasmid for cloning step after PCR	Products in use	08/03
P2	[http://partsregistry.org/Part:BBa_J61002 Bba_J61002]	Plasmid backbone, it allows us to change promoter RFP as promoter	Products in use	08/03
P3	[http://partsregistry.org/Part:BBa_J32015 Bba_J32015]	PelB leader sequence	Products in use	08/03
P4	[http://partsregistry.org/Part:BBa_B0014 Bba_B0014]	Double terminator bidirectionnal (B0012-B0011)	Products in use	08/03
P5	[http://partsregistry.org/Part:BBa_I14032 Bba_I14032]	pLac	Products in use	08/03
P6	[http://partsregistry.org/Part:BBa_K103006 BBa_K103006]	OmpA linker (464bp)	Disposed	08/03
P7	[http://partsregistry.org/Part:BBa_R0040 Bba_R0040]	TetR repressible promoter	Products in use	08/03
P8	[http://partsregistry.org/Part:BBa_I0500 Bba_I0500]	pBad/araC	Products in use	08/03
P9	ΔN-Syn1A	???	Products in use	08/03
P10	His6-SNAP25	???	Products in use	08/03
P11	VAMP2-HiB6	???	Products in use	08/03
P12	g3p	g3p in pHEN2 plasmid	Products in use	08/04
P13	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	High copy BioBrick assembly plasmid	Products in use	08/06
P14	[http://partsregistry.org/Part:BBa_K136050 BBa_K136050]	RBS-tetR	Products in use	08/06
P15	[http://partsregistry.org/Part:pSB2K3 pSB2K3]	plasmid backbone	All have been digested and purified	08/07
P16	[http://partsregistry.org/Part:pSB1A3 pSB1A3]	plasmid backbone	All have been digested and purified	08/07
P17	[http://partsregistry.org/Part:BBa_J61002 BBa_J61002]	plasmid backbone with RFP	All have been digested and purified	08/07
P18	[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]	ptet	All have been digested and purified	08/07
P19	[http://partsregistry.org/Part:BBa_K136050 BBa_K136050]	RBS-tet	All have been digested and purified	08/07
P20	[http://partsregistry.org/Part:BBa_J24679 BBa_J24679]	RBS-lacI	...	08/08
P21	[http://partsregistry.org/Part:BBa_I14032 Bba_I14032]	pLac	Products in use	08/10
P22
P23
P24
P25
P26
P27
P28	[http://partsregistry.org/Part:BBa_K103006 BBa_K103006]	OmpA linker (464bp)	PCR @57°C+ 15s@72°C	08/14

@@ Line 1: / Line 1: @@
+<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Plasmids#bottom | Plasmids]]
 {{Template:Paris2009}}
 {{Template:Paris2009_menu}}
-==Freezer==
-<center>
+== Plasmids==
-[[Team:Paris/Freezer_Primers | Primers]] -
+<html>
-[[Team:Paris/Freezer_PCR_products | PCR products]] -
+<style type="text/css">
-[[Team:Paris/Freezer_Plasmids | Plasmids]] -
+#left-side {
-[[Team:Paris/Freezer_Digestion_Products | Digestion Products]] -
+    position: absolute;
-[[Team:Paris/Freezer_Unexpected_digestion_results | Unexpected digestion results]] -
+    height: 23px;
-[[Team:Paris/Freezer_Strains_(Glycerol stock) | Strains (Glycerol stock)]]
+    width: 30px;
+    top: 0px;
+    left: 10px;
+    margin-top:10px;
+    padding-top: 7px;
+    background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png);
+    z-index:4;
+}
+#middle-side {
+    height: 25px;
+    width: 650px;
+    position: absolute;
+    top: 0px;
+    left: 30px;
+    margin-top:10px;
+    padding-top: 5px;
+    background: #dadada;
+    z-index:5;
+}
+#right-side {
+    position: absolute;
+    height: 23px;
+    width: 30px;
+    margin-top:10px;
+    padding-top: 7px;
+    top: 0px;
+    left: 670px;
+    background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png);
+    z-index:4;
+}
+a.menu_sub {
+    padding-left: 7px;
+    padding-right: 7px;
+}
+a.menu_sub_active {
+    padding-left: 7px;
+    padding-right: 7px;
+    color:#b0310e;
+    font-weight:bold;
+}
+</style>
+<div id="left-side"></div>
+<div id="middle-side"><center>
+<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>|
+<a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>|
+<a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>|
+<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>|
+<a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>|
+<a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a>
 </center>
+</div>
+<div id="right-side"></div>
+</html>
 <br>
 {|
@@ Line 26: / Line 84: @@
 |width=10% style="background: #FF0066;"| Chloranphenicol
 |}
-<html>
-</div>
-<div id="paris_content_boxtop">
-</div>
-<div id="paris_content">
-</html>
-== Plasmids: MiniPrep Products ==
 {|
 |- style="background: #c5c5c5;"
@@ Line 44: / Line 96: @@
 |width=5%| Date
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P1
+|width=5%| P1 <span/ id="P1">
 |width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
 |width=50%| General plasmid for cloning step after PCR
@@ Line 50: / Line 102: @@
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P2
+|width=5%| P2 <span/ id="P2">
-|width=25%| [http://partsregistry.org/Part:BBa_J61002 Bba-J61002]
+|width=25%| [http://partsregistry.org/Part:BBa_J61002 Bba_J61002]
 |width=50%| Plasmid backbone, it allows us to change promoter RFP as promoter
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P3
+|width=5%| P3 <span/ id="P3">
-|width=25%| [http://partsregistry.org/Part:BBa_J32015 Bba-J32015]
+|width=25%| [http://partsregistry.org/Part:BBa_J32015 Bba_J32015]
 |width=50%| PelB leader sequence
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P4
+|width=5%| P4 <span/ id="P4">
-|width=25%| [http://partsregistry.org/Part:BBa_B0014 Bba-B0014]
+|width=25%| [http://partsregistry.org/Part:BBa_B0014 Bba_B0014]
 |width=50%| Double terminator bidirectionnal (B0012-B0011)
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P5
+|width=5%| P5 <span/ id="P5">
-|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba-I14032]
+|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032]
 |width=50%| pLac
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P6
+|width=5%| P6 <span/ id="P6">
 |width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006]
 |width=50%| OmpA linker (464bp)
-|width=15%| Products in use
+|width=15%| Disposed
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P7
+|width=5%| P7 <span/ id="P7">
-|width=25%| [http://partsregistry.org/Part:BBa_R0040 Bba-R0040]
+|width=25%| [http://partsregistry.org/Part:BBa_R0040 Bba_R0040]
 |width=50%| TetR repressible promoter
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P8
+|width=5%| P8 <span/ id="P8">
-|width=25%| [http://partsregistry.org/Part:BBa_I0500 Bba-I0500]
+|width=25%| [http://partsregistry.org/Part:BBa_I0500 Bba_I0500]
 |width=50%| pBad/araC
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P9
+|width=5%| P9 <span/ id="P9">
 |width=25%| &Delta;N-Syn1A
 |width=50%| ???
@@ Line 98: / Line 150: @@
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P10
+|width=5%| P10 <span/ id="P10">
 |width=25%| His6-SNAP25
 |width=50%| ???
@@ Line 104: / Line 156: @@
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P11
+|width=5%| P11 <span/ id="P11">
 |width=25%| VAMP2-HiB6
 |width=50%| ???
@@ Line 110: / Line 162: @@
 |width=5%| [https://2009.igem.org/Team:Paris/3_August_2009 08/03]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P12
+|width=5%| P12 <span/ id="P12">
 |width=25%| g3p
 |width=50%| g3p in pHEN2 plasmid
@@ Line 116: / Line 168: @@
 |width=5%| [https://2009.igem.org/Team:Paris/4_August_2009 08/04]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P13
+|width=5%| P13 <span/ id="P13">
-|width=25%| pSB1A3
+|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
-|width=50%| pSB1A3
+|width=50%| High copy BioBrick assembly plasmid
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06]
 |- style="background: #ccccff; text-align: center;"
-|width=5%| P14
+|width=5%| P14 <span/ id="P14">
 |width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050]
-|width=50%| ???
+|width=50%| RBS-tetR
 |width=15%| Products in use
 |width=5%| [https://2009.igem.org/Team:Paris/6_August_2009 08/06]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P15 <span/ id="P15">
+|width=25%| [http://partsregistry.org/Part:pSB2K3 pSB2K3]
+|width=50%| plasmid backbone
+|width=15%| All have been digested and purified
+|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P16 <span/ id="P16">
+|width=25%| [http://partsregistry.org/Part:pSB1A3 pSB1A3]
+|width=50%| plasmid backbone
+|width=15%| All have been digested and purified
+|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P17 <span/ id="P17">
+|width=25%| [http://partsregistry.org/Part:BBa_J61002 BBa_J61002]
+|width=50%| plasmid backbone with RFP
+|width=15%| All have been digested and purified
+|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P18 <span/ id="P18">
+|width=25%| [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
+|width=50%| ptet
+|width=15%| All have been digested and purified
+|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P19 <span/ id="P19">
+|width=25%| [http://partsregistry.org/Part:BBa_K136050 BBa_K136050]
+|width=50%| RBS-tet
+|width=15%| All have been digested and purified
+|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P20 <span/ id="P20">
+|width=25%| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
+|width=50%| RBS-lacI
+|width=15%| ...
+|width=5%| [https://2009.igem.org/Team:Paris/8_August_2009 08/08]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P21 <span/ id="P21">
+|width=25%| [http://partsregistry.org/Part:BBa_I14032 Bba_I14032]
+|width=50%| pLac
+|width=15%| Products in use
+|width=5%| [https://2009.igem.org/Team:Paris/10_August_2009 08/10]
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P22 <span/ id="P22">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P23 <span/ id="P23">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P24 <span/ id="P24">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P25 <span/ id="P25">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P26 <span/ id="P26">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P27 <span/ id="P27">
+|width=25%|
+|width=50%|
+|width=15%|
+|width=5%|
+|- style="background: #ccccff; text-align: center;"
+|width=5%| P28 <span/ id="P28">
+|width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006]
+|width=50%| OmpA linker (464bp)
+|width=15%| PCR @57°C+ 15s@72°C
+|width=5%| [https://2009.igem.org/Team:Paris/14_August_2009 08/14]
 |}