Team:Paris/Freezer Primers

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Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress

Color code for sticker
Vector	Insert	PCR product	Plasmid	Strains	Kanamycin	Ampicilin	Chloranphenicol

Primers

[O1] Rev P1
Description	tolR
Sequence (5' > 3')	TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O2] Fwd P2 (ERROR : Do not use)
Description	tolR
Sequence (5' > 3')	TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O3] Rev P3
Description	tolA
Sequence (5' > 3')	TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O4] Rev P4 (ERROR: Not designed for N-term fusion)
Description	tolA
Sequence (5' > 3')	TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O5] Rev P5
Description	tatABC
Sequence (5' > 3')	TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[06] Fwd P6 (ERROR: without RBS)
Description	tatABC
Sequence (5' > 3')	TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O7] Rev P7 (ERROR: not the good standard for fusion)
Description	clyA fusion
Sequence (5' > 3')	TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O8] Fwd P8 (ERROR: No poly-Gly linker)
Description	clyA fusion
Sequence (5' > 3')	TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA
Designer	D. Bikard
Status	Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes (07/27)

[O9] clyA_Rw
Description	clyA (for N term fusion)
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: no

[O10] clyA_fusion_polyG_Rw
Description	clyA fusion (with 5-Gly linker)
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O11] ompA_signal_Fw
Description	ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O12] ompA_signal_fusion_Rw
Description	ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O13] tolR_II_Fusion_Fw
Description	For fusion of tolR domain II (standard 23)
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O14] tatABC_RBS_Fw
Description	Amplification from tatA - contains RBS
Sequence (5' > 3')	TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O15] TE3_fusion_Fw
Description	Fusion of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC
Designer	C.Loy
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O16] TE3_Rw
Description	Amplification of N-term domain of colicin E3 (standard 23)
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG
Designer	C.Loy
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O17] Tg3p_Rw
Description	Amplification of N-term domain of M13's g3p
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC
Designer	C. Loy
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O18] Tg3p_fusion_Fw
Description	Fusion of N-term domain of M13's g3p (standard 23)
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG
Designer	C.Loy
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O19] g3p_F
Description	g3p
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG
Designer	G. Beauclair
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O20] g3p_R
Description	g3p
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG
Designer	G. Beauclair
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O21] Ompa_linker_F
Description	For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3')	TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG
Designer	G. Beauclair
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O22] Ompa_linker_R
Description	For standardisation of OmpA linker (BBaK103006)
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC
Designer	G. Beauclair
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O23] OmpAsignal-TE3_seq_Fw
Description	For sequencing of part OmpAsignal-TE3
Sequence (5' > 3')	CACAAATAGCGAAAGATGAC
Designer	C.Loy
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O24] OmpAsignal-TE3_seq_Rw
Description	For sequencing of part OmpAsignal-TE3
Sequence (5' > 3')	ATCATCTGCGGGTAATGATG
Designer	C.Loy
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O25] VAMP2_Seq_Fw
Description	For sequencing of VAMP2 (snare)
Sequence (5' > 3')	ACTCACTATAGGGAGACCACA
Designer	S.Boumahdi
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O26] VAMP2_Seq_Rv
Description	For sequencing of VAMP2 (snare)
Sequence (5' > 3')	GGGCTTTGTTAGCAGCCGGAT
Designer	S.Boumahdi
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O27] ClyA_seq_Fw
Description	For sequencing of clyA after amplification
Sequence (5' > 3')	GCAGCTATTTCCAGTCACAG
Designer	V.Du
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O28] ClyA_seq_Rw
Description	For sequencing of clyA after amplification
Sequence (5' > 3')	CGCCCGCAGCAATAGAATAG
Designer	V.Du
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O29] PFecA_Fw
Description	For signal transduction
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC
Designer	R.Bodinier
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O30] PFecA_Rv
Description	For signal transduction
Sequence (5' > 3')	GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT
Designer	R.Bodinier
Status	Ordered: 07/31 ; Received: 08/07 ; Used in wet lab: no

[O31] clyA_RBS_Fw
Description	amplify clyA with RBS in the primer (std 10)
Sequence (5' > 3')	TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes (08/05)

[O32] clyA fusion_polyG_Fw
Description	clyA fusion Fw (with 5-Gly linker)
Sequence (5' > 3')	GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGCGGCGGCGGCACTGAAATCGTTGCAGATAAAAC
Designer	G. Cambray
Status	Ordered: 07/27 ; Received: no ; Used in wet lab: no ( )

@@ Line 1: / Line 1: @@
+<span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Primers#bottom | Primers]]
 {{Template:Paris2009}}
 {{Template:Paris2009_menu}}
@@ Line 10: / Line 11: @@
 [[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] -
 [[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] -
-[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]]
+[[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] - [[Team:Paris/constructions#Freezer | Construction in progress]]
 </center>
-<br>
 {|
 |- style="background: #c5c5c5;"
@@ Line 35: / Line 35: @@
 == Primers ==
 {|
-! colspan="6" style="background: #c5c5c5;" | <span/ id="PCR"> [O1] Rev P1
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O1"> [O1] Rev P1
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 52: / Line 52: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O2] Fwd P2 (ERROR : Do not use)
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O2"> [O2] Fwd P2 (ERROR : Do not use)
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 64: / Line 64: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O3] Rev P3
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O3"> [O3] Rev P3
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 81: / Line 81: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O4] Rev P4 (ERROR: Not designed for N-term fusion)
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O4"> [O4] Rev P4 (ERROR: Not designed for N-term fusion)
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 98: / Line 98: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O5] Rev P5
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O5"> [O5] Rev P5
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 115: / Line 115: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [06] Fwd P6 (ERROR: without RBS)
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O6"> [06] Fwd P6 (ERROR: without RBS)
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 132: / Line 132: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O7] Rev P7 (ERROR: not the good standard for fusion)
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O7"> [O7] Rev P7 (ERROR: not the good standard for fusion)
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 149: / Line 149: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O8] Fwd P8 (ERROR: No poly-Gly linker)
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O8"> [O8] Fwd P8 (ERROR: No poly-Gly linker)
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 166: / Line 166: @@
 |- style="background: #cbcee0;"
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
-| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#O1 07/27])
+| style="font-weight:bold;" |Ordered: 03/07 ; Received: 07/10 ; Used in wet lab: yes ([https://2009.igem.org/Team:Paris/27_July_2009#PCR 07/27])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O9] clyA_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O9"> [O9] clyA_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 189: / Line 189: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O10] clyA_fusion_polyG_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O10"> [O10] clyA_fusion_polyG_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 208: / Line 208: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O11] ompA_signal_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O11"> [O11] ompA_signal_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 221: / Line 221: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
@@ Line 227: / Line 227: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O12] ompA_signal_fusion_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O12"> [O12] ompA_signal_fusion_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 240: / Line 240: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O13] tolR_II_Fusion_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O13"> [O13] tolR_II_Fusion_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 258: / Line 258: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O14] tatABC_RBS_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O14"> [O14] tatABC_RBS_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 275: / Line 275: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O15] TE3_fusion_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O15"> [O15] TE3_fusion_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 293: / Line 293: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O16] TE3_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O16"> [O16] TE3_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 311: / Line 311: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
@@ Line 317: / Line 317: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O17] Tg3p_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O17"> [O17] Tg3p_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 330: / Line 330: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O18] Tg3p_fusion_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O18"> [O18] Tg3p_fusion_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 348: / Line 348: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
 |}
 {|
-! colspan="6" style="background: #c5c5c5;" | [O19] g3p_F
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O19"> [O19] g3p_F
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 370: / Line 370: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O20] g3p_R
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O20"> [O20] g3p_R
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 386: / Line 386: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O21] Ompa_linker_F
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O21"> [O21] Ompa_linker_F
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 403: / Line 403: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O22] Ompa_linker_R
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O22"> [O22] Ompa_linker_R
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 420: / Line 420: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O23] OmpAsignal-TE3_seq_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O23"> [O23] OmpAsignal-TE3_seq_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 437: / Line 437: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O24] OmpAsignal-TE3_seq_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O24"> [O24] OmpAsignal-TE3_seq_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 454: / Line 454: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O25] VAMP2_Seq_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O25"> [O25] VAMP2_Seq_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 471: / Line 471: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O26] VAMP2_Seq_Rv
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O26"> [O26] VAMP2_Seq_Rv
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 488: / Line 488: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O27] ClyA_seq_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O27"> [O27] ClyA_seq_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 505: / Line 505: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O28] ClyA_seq_Rw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O28"> [O28] ClyA_seq_Rw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 522: / Line 522: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O29] PFecA_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O29"> [O29] PFecA_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 539: / Line 539: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O30] PFecA_Rv
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O30"> [O30] PFecA_Rv
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 556: / Line 556: @@
 {|
-! colspan="6" style="background: #c5c5c5;" | [O31] clyA_RBS_Fw
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O31"> [O31] clyA_RBS_Fw
 |- style="background: #cbcee0;"
 |style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
@@ Line 569: / Line 569: @@
 |style="background: #0d3e99; color:white;  text-align: right;" | Status
 | style="font-weight:bold;" |Ordered: 07/27 ; Received: 08/05 ; Used in wet lab: yes
-([https://2009.igem.org/Team:Paris/5_August_2009#A1 08/05])
+([https://2009.igem.org/Team:Paris/5_August_2009#PCR 08/05])
+|}
+{|
+! colspan="6" style="background: #c5c5c5;" | <span/ id="O32"> [O32] clyA fusion_polyG_Fw
+|- style="background: #cbcee0;"
+|style="background:#0d3e99; color:white; text-align: right;" width=120px|Description
+|width=700px| clyA fusion Fw (with 5-Gly linker)
+|- style="background: #cbcee0;"
+|style="background:#0d3e99; color:white; text-align: right;"|Sequence (5' > 3')
+|GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGCGGCGGCGGCGGCACTGAAATCGTTGCAGATAAAAC
+|- style="background: #cbcee0;"
+|style="background:#0d3e99; color:white; text-align: right;"|Designer
+|G. Cambray
+|- style="background: #cbcee0;"
+|style="background: #0d3e99; color:white;  text-align: right;" | Status
+| style="font-weight:bold;" |Ordered: 07/27 ; Received: no ; Used in wet lab: no
+( )
 |}