Team:Paris/Freezer Plasmids
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+ | <span/ id="bottom">[https://2009.igem.org/ iGEM ] > [[Team:Paris#top | Paris]] > [[Team:Paris/Freezer#top | Freezer]] > [[Team:Paris/Freezer_Plasmids#bottom | Plasmids]] | ||
{{Template:Paris2009}} | {{Template:Paris2009}} | ||
{{Template:Paris2009_menu}} | {{Template:Paris2009_menu}} | ||
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- | <center> | + | == Plasmids== |
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- | + | <style type="text/css"> | |
- | + | #left-side { | |
- | + | position: absolute; | |
- | + | height: 23px; | |
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+ | top: 0px; | ||
+ | left: 10px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/1/1b/Left_menu_pari.png); | ||
+ | z-index:4; | ||
+ | } | ||
+ | |||
+ | #middle-side { | ||
+ | height: 25px; | ||
+ | width: 650px; | ||
+ | position: absolute; | ||
+ | top: 0px; | ||
+ | left: 30px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 5px; | ||
+ | background: #dadada; | ||
+ | z-index:5; | ||
+ | } | ||
+ | |||
+ | #right-side { | ||
+ | position: absolute; | ||
+ | height: 23px; | ||
+ | width: 30px; | ||
+ | margin-top:10px; | ||
+ | padding-top: 7px; | ||
+ | top: 0px; | ||
+ | left: 670px; | ||
+ | background: url(https://static.igem.org/mediawiki/2009/4/40/Right_menu_paris.png); | ||
+ | z-index:4; | ||
+ | } | ||
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+ | a.menu_sub { | ||
+ | padding-left: 7px; | ||
+ | padding-right: 7px; | ||
+ | } | ||
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+ | a.menu_sub_active { | ||
+ | padding-left: 7px; | ||
+ | padding-right: 7px; | ||
+ | color:#b0310e; | ||
+ | font-weight:bold; | ||
+ | } | ||
+ | </style> | ||
+ | <div id="left-side"></div> | ||
+ | <div id="middle-side"><center> | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer#bottom"> Main </a>| | ||
+ | <a class="menu_sub" href="https://2009.igem.org/Team:Paris/Freezer_PCR_products#bottom"> PCR Products</a>| | ||
+ | <a class="menu_sub_active"href="https://2009.igem.org/Team:Paris/Freezer_Plasmids#bottom"> Plasmids</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Digestion_Products#bottom"> Digestion Products</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#bottom"> Strains (Glycerol stock)</a>| | ||
+ | <a class="menu_sub"href="https://2009.igem.org/Team:Paris/constructions#bottom"> Constructions in progress</a> | ||
</center> | </center> | ||
+ | </div> | ||
+ | |||
+ | <div id="right-side"></div> | ||
+ | |||
+ | </html> | ||
+ | |||
<br> | <br> | ||
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Latest revision as of 15:27, 19 October 2009
iGEM > Paris > Freezer > Plasmids
Plasmids
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Plasmids: MiniPrep Products | ||||
---|---|---|---|---|
Number | Name | Description | Products in use | Date |
P1 | [http://partsregistry.org/Part:pSB2K3 pSB2K3] | General plasmid for cloning step after PCR | Products in use | 08/03 |
P2 | [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] | Plasmid backbone, it allows us to change promoter RFP as promoter | Products in use | 08/03 |
P3 | [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] | PelB leader sequence | Products in use | 08/03 |
P4 | [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] | Double terminator bidirectionnal (B0012-B0011) | Products in use | 08/03 |
P5 | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] | pLac | Products in use | 08/03 |
P6 | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] | OmpA linker (464bp) | Disposed | 08/03 |
P7 | [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] | TetR repressible promoter | Products in use | 08/03 |
P8 | [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] | pBad/araC | Products in use | 08/03 |
P9 | ΔN-Syn1A | ??? | Products in use | 08/03 |
P10 | His6-SNAP25 | ??? | Products in use | 08/03 |
P11 | VAMP2-HiB6 | ??? | Products in use | 08/03 |
P12 | g3p | g3p in pHEN2 plasmid | Products in use | 08/04 |
P13 | [http://partsregistry.org/Part:pSB1A3 pSB1A3] | High copy BioBrick assembly plasmid | Products in use | 08/06 |
P14 | [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | RBS-tetR | Products in use | 08/06 |
P15 | [http://partsregistry.org/Part:pSB2K3 pSB2K3] | plasmid backbone | All have been digested and purified | 08/07 |
P16 | [http://partsregistry.org/Part:pSB1A3 pSB1A3] | plasmid backbone | All have been digested and purified | 08/07 |
P17 | [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] | plasmid backbone with RFP | All have been digested and purified | 08/07 |
P18 | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | ptet | All have been digested and purified | 08/07 |
P19 | [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] | RBS-tet | All have been digested and purified | 08/07 |
P20 | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS-lacI | ... | 08/08 |
P21 | [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] | pLac | Products in use | 08/10 |
P22 | ||||
P23 | ||||
P24 | ||||
P25 | ||||
P26 | ||||
P27 | ||||
P28 | [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] | OmpA linker (464bp) | PCR @57°C+ 15s@72°C | 08/14 |