Team:TUDelft/27 August 2009

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(New page: ===Saeed=== The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment s...)
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{{Template:TUDelftiGEM2009}}
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{{Template:TUDelftiGEM2009_LabNotebook}}
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='''27 August 2009'''=
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===Sriram===
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Yesterday's gel is shown below. I continued the ligation and electro-transformation of assemblies. I will check the colonies tomorrow and I will be back to lab only on 1st September since tomorrow and 31st August I will be on leave for mentoring job and US visa interview.
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[[Image:gel270809-Delay Ribo Assembly II 5678TT.jpg|thumb|center|450px]]
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===Saeed===
===Saeed===
The overnight colonies were used for miniprep.
The overnight colonies were used for miniprep.
!Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.  
!Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.  
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[[Image:Gelsaeed27-082009_%21Z.wiki.jpg|center|450px]]
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{| border="1" align="center"
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| lane || Part || Expected Plasmid Size || Status
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|- align="center"
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| 1 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||
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|- align="center"
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| 2 || !Za  || 1934 || no band
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|- align="center"
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| 3 || !Zb || 1934 || ok
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|- align="center"
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| 4 || !Zg || 1934 || ok
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|- align="center"
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| 5 || !Zh || 1934 || ok
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|- align="center"
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| 6 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||  ||
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|}
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!Zb and !Zg seems to be ok. !Zh looks a bit bigger, but this also could be because of the gel. !Zb or !Zg should be used for sequencing.
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===Daniel===
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Miniprep of locks and keys in biobrick´s plasmid backbones and some previous assemblies
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{| border="1" align="center"
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| Part || [DNA] ng/uL
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|- align="center"
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| LMA || 46.1
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|- align="center"
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| KMA || 58.8
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|- align="center"
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| LWA || 59.1
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|- align="center"
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| KWA || 54.7
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|- align="center"
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| 2B || 64.4
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|- align="center"
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| 1A || 122.2
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|- align="center"
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| 1B || 113.3
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|}
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Restriction of Locks and keys to use them for assembly. The gel shows weird results.
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[[Image:gel280809.jpg|550px]]
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Ligation of 1C, 2C, 3C and 4C
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Latest revision as of 20:40, 20 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

27 August 2009

Sriram

Yesterday's gel is shown below. I continued the ligation and electro-transformation of assemblies. I will check the colonies tomorrow and I will be back to lab only on 1st September since tomorrow and 31st August I will be on leave for mentoring job and US visa interview.

Gel270809-Delay Ribo Assembly II 5678TT.jpg

Saeed

The overnight colonies were used for miniprep. !Za, !Zb, !Zg and !Zh had the highest DNA concentrations. These were downstream cut and checked on 1% agarose gel for fragment size. Fragment should be 1934 bp.

Gelsaeed27-082009 !Z.wiki.jpg
lane Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]
2  !Za 1934 no band
3  !Zb 1934 ok
4  !Zg 1934 ok
5  !Zh 1934 ok
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder]

!Zb and !Zg seems to be ok. !Zh looks a bit bigger, but this also could be because of the gel. !Zb or !Zg should be used for sequencing.

Daniel

Miniprep of locks and keys in biobrick´s plasmid backbones and some previous assemblies

Part [DNA] ng/uL
LMA 46.1
KMA 58.8
LWA 59.1
KWA 54.7
2B 64.4
1A 122.2
1B 113.3

Restriction of Locks and keys to use them for assembly. The gel shows weird results.

Gel280809.jpg

Ligation of 1C, 2C, 3C and 4C

Retrieved from "http://2009.igem.org/Team:TUDelft/27_August_2009"