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Team:TUDelft/25 August 2009
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'''25 August 2009''' | '''25 August 2009''' | ||
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| + | ===Sriram=== | ||
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| + | Today I got colonies in all plates for the first stage assemblies for riboregulator. I cultured colonies from plates for doing miniprep and next stage of the assembly. | ||
===Daniel=== | ===Daniel=== | ||
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Digestion of 4A, 2A and 1A | Digestion of 4A, 2A and 1A | ||
| - | [[Image:gel250809.jpg| | + | [[Image:gel250809.jpg|550px]] |
Rehydratation of oligos from BASECLEAR, PCR and purification. | Rehydratation of oligos from BASECLEAR, PCR and purification. | ||
Latest revision as of 20:16, 20 October 2009
Lab Notebook
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25 August 2009
Sriram
Today I got colonies in all plates for the first stage assemblies for riboregulator. I cultured colonies from plates for doing miniprep and next stage of the assembly.
Daniel
Assemblies 1B, 2B and 3B. Digestion of 4A, 2A and 1A
Rehydratation of oligos from BASECLEAR, PCR and purification.
| Part | [DNA] ng/uL |
| Lock for weak RBS | 100.8 |
| Key for weak RBS | 128.6 |
| Lock for medium RBS | 83.1 |
| Key for medium RBS | 112.5 |
