Team:TUDelft/7 September 2009

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='''7 September 2009'''=
='''7 September 2009'''=
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I didnt get any colonies in the locks and keys plates. Hence I ran the gel (shown below) to confirm my restricted products. Then I again did ligation and electro transformation of them. Today from weenink I understood that I need to make a positive control. It is very simple. I have to just attach the restricted biobricks pLacI and RBS-GFP-TT. Hence I did it. Sadly all our sequencing samples were failed due to double signal. We have to check the primers.
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[[Image:Delay070909.jpg|thumb|center|450px]]
===Saeed===
===Saeed===
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upstream restircted !P1 was used for assembly with downstream double terminator. After the assembly the plasmide was electro transformed. Cell are plated on medium containing CAM.
upstream restircted !P1 was used for assembly with downstream double terminator. After the assembly the plasmide was electro transformed. Cell are plated on medium containing CAM.
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Today I also count the kolonies of Tim Weeniks tranformation of Friday 04-09-2009.
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Today I also count the colonies of Tim Weeninks tranformation of Friday 04-09-2009.
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{| border="1" align="center
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| Transformation || plate number || number of colonies
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|- align="center"
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| !SIII A1 ||1 || 1 
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|- align="center"
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| !SIII A1 || 2  || 27
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|- align="center"
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| !SIII A2|| 1 || 24 
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|- align="center"
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| !SIII A2|| 2 || 6
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|- align="center"
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| !SIII A3|| 1 || 50
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|- align="center"
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| !SIII A3|| 2 || 2
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|- align="center"
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| !SIII A4|| 1 || 47
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|- align="center"
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| !SIII A4|| 2 ||150+ 
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|}
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I made 10 overnight cultures form these plates, I picked one colonie from each plate. From both A4 plates I picked two colonies.
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Latest revision as of 23:13, 21 October 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

7 September 2009

I didnt get any colonies in the locks and keys plates. Hence I ran the gel (shown below) to confirm my restricted products. Then I again did ligation and electro transformation of them. Today from weenink I understood that I need to make a positive control. It is very simple. I have to just attach the restricted biobricks pLacI and RBS-GFP-TT. Hence I did it. Sadly all our sequencing samples were failed due to double signal. We have to check the primers.

Delay070909.jpg

Saeed

upstream restircted !P1 was used for assembly with downstream double terminator. After the assembly the plasmide was electro transformed. Cell are plated on medium containing CAM.

Today I also count the colonies of Tim Weeninks tranformation of Friday 04-09-2009.

Transformation plate number number of colonies
 !SIII A1 1 1
 !SIII A1 2 27
 !SIII A2 1 24
 !SIII A2 2 6
 !SIII A3 1 50
 !SIII A3 2 2
 !SIII A4 1 47
 !SIII A4 2 150+

I made 10 overnight cultures form these plates, I picked one colonie from each plate. From both A4 plates I picked two colonies.

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