Team:TUDelft/16 September 2009
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Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. | Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. | ||
- | Hence with the correct LacI generator I ligated the control plasmids | + | Hence with the correct LacI generator I ligated the control plasmids. |
Then they were electroporated to cells. | Then they were electroporated to cells. | ||
[[Image:DelayGel-I-160909.bmp|thumb|center|450px]] | [[Image:DelayGel-I-160909.bmp|thumb|center|450px]] |
Revision as of 00:57, 22 October 2009
Lab Notebook
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16 September 2009
Sriram
Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. Hence with the correct LacI generator I ligated the control plasmids. Then they were electroporated to cells.
Tim Weenink
Ligated the following constructs: !Y and !X and transformed the E. Coli K12 strain with them.