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Team:TUDelft/8 July 2009
From 2009.igem.org
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='''8th July'''= | ='''8th July'''= | ||
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=='''Conjugation'''== | =='''Conjugation'''== | ||
Today, we worked on the entries to the Parts Registry, where we used some parts from the Peking '07 iGEM team and some parts from NCBI. After writing down the sequences for trbC (pilin) of IncP beta R751 plasmid, trbK (entry exclusion) of IncP beta R751 plasmid, I-PpoI homing endonuclease site, I-SceI homing endonuclease site and I-PpoI homing endonuclease into the registry, we used the software [http://www.vectorcore.pitt.edu/upgene/upgene.html Upgene] to optimize for the codons, and the optimized sequences were inserted into the Parts Registry. | Today, we worked on the entries to the Parts Registry, where we used some parts from the Peking '07 iGEM team and some parts from NCBI. After writing down the sequences for trbC (pilin) of IncP beta R751 plasmid, trbK (entry exclusion) of IncP beta R751 plasmid, I-PpoI homing endonuclease site, I-SceI homing endonuclease site and I-PpoI homing endonuclease into the registry, we used the software [http://www.vectorcore.pitt.edu/upgene/upgene.html Upgene] to optimize for the codons, and the optimized sequences were inserted into the Parts Registry. | ||
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Latest revision as of 14:27, 25 July 2009
Lab Notebook
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8th July
Conjugation
Today, we worked on the entries to the Parts Registry, where we used some parts from the Peking '07 iGEM team and some parts from NCBI. After writing down the sequences for trbC (pilin) of IncP beta R751 plasmid, trbK (entry exclusion) of IncP beta R751 plasmid, I-PpoI homing endonuclease site, I-SceI homing endonuclease site and I-PpoI homing endonuclease into the registry, we used the software [http://www.vectorcore.pitt.edu/upgene/upgene.html Upgene] to optimize for the codons, and the optimized sequences were inserted into the Parts Registry.
