Team:TUDelft/30 July 2009

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(Difference between revisions)
(→Tim Weenink)
Line 8: Line 8:
prepared assembly mixes for !B, !C and !D constructs.
prepared assembly mixes for !B, !C and !D constructs.
 +
Standard Ginko assembly kit was used.
 +
DNA concentrations as below (these mixtures were added to 7.5 µl of digestion mixture):
 +
{| border="1" align="center"
 +
| Assembly name || Components || volume in µl
 +
|- align="center"
 +
| !B|| Upstream || !A DNA || 33.0
 +
|- align="center"
 +
| ||  || H20 || 9.5
 +
|- align="center"
 +
| || Downstream || BBa_B0015 DNA || 10.0
 +
|- align="center"
 +
| ||  || H20 || 32.5
 +
|- align="center"
 +
| || Backbone || pSB1AC3 DNA || 6.5
 +
|- align="center"
 +
| ||  || H20 || 36
 +
 +
|- align="center"
 +
| !C|| Upstream || BBa_R0040 DNA || 10.0
 +
|- align="center"
 +
| ||  || H20 || 32.5
 +
|- align="center"
 +
| || Downstream || *I6 DNA || 5.5
 +
|- align="center"
 +
| ||  || H20 || 37.0
 +
|- align="center"
 +
| || Backbone || pSB1AC3 DNA || 6.5
 +
|- align="center"
 +
| ||  || H20 || 36
 +
|- align="center"
 +
 +
| !D|| Upstream || *I6 DNA || 5.5
 +
|- align="center"
 +
| ||  || H20 || 37.0
 +
|- align="center"
 +
| || Downstream || BBa_K145201 DNA || 18.0
 +
|- align="center"
 +
| ||  || H20 || 24.5
 +
|- align="center"
 +
| || Backbone || pSB1AC3 DNA || 6.5
 +
|- align="center"
 +
| ||  || H20 || 36
 +
|}
===Calin===
===Calin===

Revision as of 15:09, 30 July 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

30 July 2009

Tim Weenink

prepared assembly mixes for !B, !C and !D constructs. Standard Ginko assembly kit was used. DNA concentrations as below (these mixtures were added to 7.5 µl of digestion mixture):

Assembly name Components volume in µl
 !B Upstream  !A DNA 33.0
H20 9.5
Downstream BBa_B0015 DNA 10.0
H20 32.5
Backbone pSB1AC3 DNA 6.5
H20 36
 !C Upstream BBa_R0040 DNA 10.0
H20 32.5
Downstream *I6 DNA 5.5
H20 37.0
Backbone pSB1AC3 DNA 6.5
H20 36
 !D Upstream *I6 DNA 5.5
H20 37.0
Downstream BBa_K145201 DNA 18.0
H20 24.5
Backbone pSB1AC3 DNA 6.5
H20 36

Calin

Plated three backbones left over from the transformation the other day. Did a digest on oriT-R with E and P.


Tim Vos

Minipreped pSB4C5, pSB.


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