Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(→Digestion Products)
(→Digestion Products)
Line 57: Line 57:
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 66pb
| PelB leader sequence  
| PelB leader sequence  
| ???
| ???
Line 68: Line 68:
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 95pb
| Double terminator bidirectionnal (B0012-B0011)
| Double terminator bidirectionnal (B0012-B0011)
| ???
| ???
Line 79: Line 79:
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 37pb
| pLac
| pLac
| ???
| ???
Line 90: Line 90:
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 54pb
| TetR repressible promote
| TetR repressible promote
| ???
| ???
Line 101: Line 101:
| XbaI
| XbaI
| PstI
| PstI
-
| Size
+
| 1210
| pBad/araC  
| pBad/araC  
| ???
| ???

Revision as of 15:03, 5 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Product Name Matrix Name Enz. 1 Enz. 2 Size Description Product Left []ng/µL Date
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence  ???  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011)  ???  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac  ???  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote  ???  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210 pBad/araC  ???  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet).  ???  ??? 08/04
D7  ???  ??? XbaI PstI Size  ???  ???  ??? 08/04

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