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Team:Paris/Freezer Digestion Products
From 2009.igem.org
(Difference between revisions)
(→Digestion Products) |
(→Digestion Products) |
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| Line 59: | Line 59: | ||
| 66pb | | 66pb | ||
| PelB leader sequence | | PelB leader sequence | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| Line 70: | Line 70: | ||
| 95pb | | 95pb | ||
| Double terminator bidirectionnal (B0012-B0011) | | Double terminator bidirectionnal (B0012-B0011) | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| Line 81: | Line 81: | ||
| 37pb | | 37pb | ||
| pLac | | pLac | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| Line 92: | Line 92: | ||
| 54pb | | 54pb | ||
| TetR repressible promote | | TetR repressible promote | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| Line 103: | Line 103: | ||
| 1210pb | | 1210pb | ||
| pBad/araC | | pBad/araC | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
| Line 114: | Line 114: | ||
| 2053bp (and 895bp) | | 2053bp (and 895bp) | ||
| Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | ||
| - | | | + | | 47 |
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | | [https://2009.igem.org/Team:Paris/4_August_2009 08/04] | ||
Revision as of 13:24, 6 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enz. 1 | Enz. 2 | Size | Description | Product Left | []ng/µL | Date |
| D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | 66pb | PelB leader sequence | 47 | ??? | 08/04 |
| D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | 95pb | Double terminator bidirectionnal (B0012-B0011) | 47 | ??? | 08/04 |
| D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | 37pb | pLac | 47 | ??? | 08/04 |
| D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | 54pb | TetR repressible promote | 47 | ??? | 08/04 |
| D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | 1210pb | pBad/araC | 47 | ??? | 08/04 |
| D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | 47 | ??? | 08/04 |
| D7 | ??? | ??? | XbaI | PstI | Size | ??? | ??? | ??? | 08/04 |
