Team:TUDelft/5 August 2009

From 2009.igem.org

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Revision as of 18:59, 6 August 2009

Lab Notebook

July
MTWTFSS
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August
MTWTFSS
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September
MTWTFSS
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October
MTWTFSS
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5 August 2009

Tim Weenink

Tim050809.png


Tim050809!Dandcalin.png


Well Part Expected Plasmid Size Status
1-8  !D PCR products (wrong primer) 1100 ✖
9 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
10 pSB1AK3 linear 3864 ✔
11 pSB4C5 linear 3896 ✔
12 strong promoter 2983 ✔
13 oriT-R linear  ? ✔

Orr

Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.

Calin

Made test plate for Trimpethoprim.

Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.

Ran three conjugations in parallel, with a conjugation time of 1 hour.

Starting OD for recipients 0.502, for donors 0.542.

Made a total of 40 plates:


Set 1
Plate ID Antibiotics Dilution
D1 TRI 100
D2 TRI 10-2
D3 TRI 10-4
D4 TRI 10-5
D5 TRI 10-6
T1 TRI + AMP 100
T2 TRI + AMP 10-1
T3 TRI + AMP 10-2
T4 TRI + AMP 10-3
T5 TRI + AMP 10-4
T6 TRI + AMP 10-5
T7 TRI + AMP 10-6
R1 AMP 100
R2 AMP 10-4
R3 AMP 10-5
R4 AMP 10-6



Set 2
Plate ID Antibiotics Dilution
D6 TRI 100
D7 TRI 10-2
D8 TRI 10-4
D9 TRI 10-5
D10 TRI 10-6
T8 TRI + AMP 100
T9 TRI + AMP 10-1
T10 TRI + AMP 10-2
T11 TRI + AMP 10-3
T12 TRI + AMP 10-4
T13 TRI + AMP 10-5
T14 TRI + AMP 10-6



Set 3
Plate ID Antibiotics Dilution
D11 TRI 100
D12 TRI 10-2
D13 TRI 10-4
D14 TRI 10-5
D15 TRI 10-6
T15 TRI + AMP 100
T16 TRI + AMP 10-1
T17 TRI + AMP 10-2
T18 TRI + AMP 10-3
T19 TRI + AMP 10-4
T20 TRI + AMP 10-5
T21 TRI + AMP 10-6

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