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Team:Paris/4 August 2009
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*Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | *Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | ||
| - | D1=P3, D2=P4, D3=P5, D4=P7, D5=P8, (D4 seems to be bad because we don't see is specific band during a gel after is purification) | + | D1=[P3[https://2009.igem.org/Team:Paris/Freezer_Plasmids]]Digested, D2=P4, D3=P5, D4=P7, D5=P8, (D4 seems to be bad because we don't see is specific band during a gel after is purification) |
**P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow | **P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow | ||
*Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | *Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | ||
Revision as of 13:13, 7 August 2009
Contents |
NoteBook
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Brain work
A lot of meeting today: First on for the modeling explication and application of stochastic model ? The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa
Lab work
Overnight culture
- Keio Collection
- [S20] JW4251: LB Kan1000x 200rpm 37°C
- [S21] JN0729: LB Kan1000x 200rpm 37°C
- [S22] JN0728: LB Kan1000x 200rpm 37°C
- [S23] JN0727: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 37°C
- [S25] JW5100: LB Kan1000x 200rpm 37°C
- [S25] JW5100: LB Kan1000x 37°C
- [S26] JN5181: LB Kan1000x 200rpm 37°C
- [S27] FF64: LB Tet1000x 200rpm 37°C
- [S28] NEC280: LB 200rpm 37°C
- [S5] MG4: LB Kan NaCl20% 200rpm 37°C
- [S6] DH5α: LB 200rpm 37°C
- [S24] JN0940: LBagar plate with Kan1000x 37°C
- [S25] JW5100: LBagar plate with Kan1000x 37°C
Biobrick resuspention
- 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
Transformation of precited Biobrick
- 1K1 (pSB1A3) in competent DH5α by RbCl [Protocol]
Purification
- Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
D1=[P3[1]]Digested, D2=P4, D3=P5, D4=P7, D5=P8, (D4 seems to be bad because we don't see is specific band during a gel after is purification)
- P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
- Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
To do list
| Matricule | TODO |
| Luc | SNARE Construction |
| Romain | Design oligo Fec A |
| Charlotte | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
| Stoff | Dev schedule algorithm / BDD |
| Chris | modeling : we don't want fu*king oscillations ! |
| Lisa | Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting |
| Caroline | coloration of cells / protocols for the lab: control of the emission of vesicules.... |
| Souf | oligos desing / Labs |
| Vicard | Gel / SNARE |
| Pierre | modeling : we don't want fu*king oscillations ! |
| Sylvain | minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise |
| Guillaume | Lab/ g3p |
