Team:Paris/4 August 2009
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*Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | *Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb). | ||
- | D1=[ | + | D1=[[https://2009.igem.org/Team:Paris/Freezer_Plasmids P3]]Digested=66pb, D2=[[https://2009.igem.org/Team:Paris/Freezer_Plasmids P4]]Digested=95pb, D3=[[https://2009.igem.org/Team:Paris/Freezer_Plasmids P5]]Digested=37pb, D4=[[https://2009.igem.org/Team:Paris/Freezer_Plasmids P7]]Digested=54pb, D5=[[https://2009.igem.org/Team:Paris/Freezer_Plasmids P8]]Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification) |
- | + | *P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow | |
*Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | *Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8] | ||
<center>[[Image:4aout1er_o.JPG]]</center> | <center>[[Image:4aout1er_o.JPG]]</center> | ||
+ | *D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands) | ||
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Revision as of 13:24, 7 August 2009
Contents |
NoteBook
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Brain work
A lot of meeting today: First on for the modeling explication and application of stochastic model ? The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa
Lab work
Overnight culture
- Keio Collection
- [S20] JW4251: LB Kan1000x 200rpm 37°C
- [S21] JN0729: LB Kan1000x 200rpm 37°C
- [S22] JN0728: LB Kan1000x 200rpm 37°C
- [S23] JN0727: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 37°C
- [S25] JW5100: LB Kan1000x 200rpm 37°C
- [S25] JW5100: LB Kan1000x 37°C
- [S26] JN5181: LB Kan1000x 200rpm 37°C
- [S27] FF64: LB Tet1000x 200rpm 37°C
- [S28] NEC280: LB 200rpm 37°C
- [S5] MG4: LB Kan NaCl20% 200rpm 37°C
- [S6] DH5α: LB 200rpm 37°C
- [S24] JN0940: LBagar plate with Kan1000x 37°C
- [S25] JW5100: LBagar plate with Kan1000x 37°C
Biobrick resuspention
- 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
Transformation of precited Biobrick
- 1K1 (pSB1A3) in competent DH5α by RbCl [Protocol]
Purification
- Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
D1=[P3]Digested=66pb, D2=[P4]Digested=95pb, D3=[P5]Digested=37pb, D4=[P7]Digested=54pb, D5=[P8]Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
- P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
- Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
- D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)
To do list
Matricule | TODO |
Luc | SNARE Construction |
Romain | Design oligo Fec A |
Charlotte | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
Stoff | Dev schedule algorithm / BDD |
Chris | modeling : we don't want fu*king oscillations ! |
Lisa | Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting |
Caroline | coloration of cells / protocols for the lab: control of the emission of vesicules.... |
Souf | oligos desing / Labs |
Vicard | Gel / SNARE |
Pierre | modeling : we don't want fu*king oscillations ! |
Sylvain | minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise |
Guillaume | Lab/ g3p |