Team:Paris/27 July 2009
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*Media: 15 LB agarosis ampiciline dishes were made. | *Media: 15 LB agarosis ampiciline dishes were made. | ||
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*PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion). | *PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion). | ||
**Matrice is Genomic DNA of [S2] K12 1655 ΔrecA::Kan. [[https://2009.igem.org/Team:Paris/Protocols_PCRqload#PCR_with_Quick_load_Taq2x_Master_Mix Protocol]]. | **Matrice is Genomic DNA of [S2] K12 1655 ΔrecA::Kan. [[https://2009.igem.org/Team:Paris/Protocols_PCRqload#PCR_with_Quick_load_Taq2x_Master_Mix Protocol]]. |
Revision as of 10:10, 9 August 2009
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Lab work
- Media: 15 LB agarosis ampiciline dishes were made.
- PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion).
- Matrice is Genomic DNA of [S2] K12 1655 ΔrecA::Kan. [Protocol].
- PCR launched with a Tm at 60°C (programme quick load).
- Buffer for Competent bacteria by RbCl
- [F9] Buffer I (250ml)
- [F10] Buffer II (125ml)
- DH5 alpha competent bacteria by RbCl [Protocol]