Team:Paris/constructions

From 2009.igem.org

(Difference between revisions)
(Constructions)
(Constructions)
Line 34: Line 34:
== Constructions ==
== Constructions ==
-
 
-
*'''Plasmid B'''
 
-
 
-
 
-
{|
 
-
|- style="background: #0d3e99; text-align: center; color:white;"
 
-
|width=3%| Designer
 
-
|width=40%| Parts description
 
-
|width=25%| Corresponding BioBricks
 
-
|width=25%| Status (last update)
 
-
|width=3%| Plasmid #
 
-
|width=4%| Registry
 
-
 
-
|- style="background: #ccccff; text-align: center"
 
-
|GC
 
-
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
 
-
|BBa_blabla :: BBa_tralala in truc
 
-
|Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09)
 
-
|12
 
-
|BBa_pouet pouet
 
-
 
-
|- style="background: #ccff00; text-align: center"
 
-
|CC
 
-
|Plac :: rbs-tetR in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #ccff00; text-align: center"
 
-
|CC
 
-
|(Plac :: rbs-tetR) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #ccff00; text-align: center"
 
-
|CC
 
-
|ter :: ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #00FF00; text-align: center"
 
-
|CC
 
-
|Ptet :: (rbs - OmpAsignal :: TolRII) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #00FF00; text-align: center"
 
-
|CC
 
-
|rbs :: EYFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #00FF00; text-align: center"
 
-
|CC
 
-
|Ptet :: (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #006600; text-align: center"
 
-
|CC
 
-
|(Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|- style="background: #006600; text-align: center"
 
-
|CC
 
-
|(Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 
-
|
 
-
|??
 
-
|BBa_????
 
-
 
-
|}
 
Line 258: Line 172:
|BBa_????
|BBa_????
 +
 +
|}
 +
 +
 +
*'''Plasmid B'''
 +
 +
 +
{|
 +
|- style="background: #0d3e99; text-align: center; color:white;"
 +
|width=3%| Designer
 +
|width=40%| Parts description
 +
|width=25%| Corresponding BioBricks
 +
|width=25%| Status (last update)
 +
|width=3%| Plasmid #
 +
|width=4%| Registry
 +
 +
|- style="background: #ccccff; text-align: center"
 +
|GC
 +
|((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3
 +
|BBa_blabla :: BBa_tralala in truc
 +
|Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09)
 +
|12
 +
|BBa_pouet pouet
 +
 +
|- style="background: #ccff00; text-align: center"
 +
|CC
 +
|Plac :: rbs-tetR in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #ccff00; text-align: center"
 +
|CC
 +
|(Plac :: rbs-tetR) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #ccff00; text-align: center"
 +
|CC
 +
|ter :: ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|[http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #00FF00; text-align: center"
 +
|CC
 +
|Ptet :: (rbs - OmpAsignal :: TolRII) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #00FF00; text-align: center"
 +
|CC
 +
|rbs :: EYFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #00FF00; text-align: center"
 +
|CC
 +
|Ptet :: (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|[http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #006600; text-align: center"
 +
|CC
 +
|(Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
 +
 +
|- style="background: #006600; text-align: center"
 +
|CC
 +
|(Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3]
 +
|
 +
|??
 +
|BBa_????
|}
|}

Revision as of 16:42, 10 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Constructions

  • Plasmid A


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
CC Pbad :: rbs - lacI - LVA in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (Pbad :: rbs - lacI - LVA) :: RFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K093005 BBa_K093005] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC ((Pbad :: rbs - lacI - LVA) :: RFP) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC OmpA Linker :: g3p in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (OmpA Linker :: g3p) :: ter in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (rbs - june/foss) :: ter in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (rbs - fecA) :: ((rbs - jun/foss) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (rbs - fecA) :: ((Ompa Linker :: g3p) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????


CC (rbs - ClyA-GFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (rbs - ClyA-GFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC Pbad :: ((rbs - ClyA-GFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC Pbad :: ((rbs - ClyA-GFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter)) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (Pbad :: ((rbs - ClyA-GFP) :: (((rbs - fecA) :: (OmpA linker :: g3p)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????
CC (Pbad :: ((rbs - ClyA-GFP) :: (((rbs - fecA) :: (rbs - jun/foss)) :: ter))) :: ((Pbad :: rbs - lacI - LVA) :: ter) in [http://partsregistry.org/Part:pSB2K3 pSB2K3] BBa_??? :: BBa_???? in [http://partsregistry.org/Part:pSB2K3 pSB2K3] ?? BBa_????



  • Plasmid B


Designer Parts description Corresponding BioBricks Status (last update) Plasmid # Registry
GC ((Plac :: rbs-tetR) :: rbs-tolRII) in pSB2A3 BBa_blabla :: BBa_tralala in truc Planning / oligo desgned or ordered / PCR in progress / cloned / sequencing... (10/08/09) 12 BBa_pouet pouet
CC Plac :: rbs-tetR in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (Plac :: rbs-tetR) :: ter in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_???? :: [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC ter :: ((Plac :: rbs-tetR) :: ter) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC Ptet :: (rbs - OmpAsignal :: TolRII) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC rbs :: EYFP in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0030 BBa_E0030] in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC Ptet :: (rbs :: EYFP) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (Ptet :: (rbs :: EYFP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????
CC (Ptet :: (rbs - OmpAsignal :: TolRIIP)) :: (ter :: ((Plac :: rbs-tetR) :: ter)) in [http://partsregistry.org/Part:pSB1A3 pSB1A3] BBa_??? :: BBa_??? in [http://partsregistry.org/Part:pSB1A3 pSB1A3] ?? BBa_????