Team:TUDelft/18 August 2009

From 2009.igem.org

(Difference between revisions)
(→18 August 2009)
(→Calin)
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| 19 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||   || <font color=limegreen>&#10004;</font>
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| 20 || [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ||   || <font color=limegreen>&#10004;</font>
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| 26 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||  
| 26 || Colony PCR R751 + pKD46 + oriTR_KO_mod_S  || 398 ||  
|- align="center"
|- align="center"
 +
|}
 +
 +
 +
For the trbK conjugation test 24 plates were made:
 +
 +
 +
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 1</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D1tr || TRI || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D2tr || TRI || 10<sup>-1</sup>||
 +
|- align="center"
 +
| D3tr || TRI || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D4tr || TRI || 10<sup>-3</sup>||
 +
|- align="center"
 +
| D5tr || TRI || 10<sup>-4</sup>||
 +
|- align="center"
 +
| T1tr || TRI + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| T2tr || TRI + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| T3tr || TRI + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| T4tr || TRI + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| T5tr || TRI + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R1tr || CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R2tr || CAM || 10<sup>-1</sup>||
 +
|}
 +
 +
<br><br>
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 2</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D6tr || TRI || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D7tr || TRI || 10<sup>-1</sup>||
 +
|- align="center"
 +
| D8tr || TRI || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D9tr || TRI || 10<sup>-3</sup>||
 +
|- align="center"
 +
| D10tr || TRI || 10<sup>-4</sup>||
 +
|- align="center"
 +
| T6tr || TRI + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| T7tr || TRI + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| T8tr || TRI + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| T9tr || TRI + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| T10tr || TRI + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R3tr || CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R4tr || CAM || 10<sup>-1</sup>||
 +
|}
 +
 +
For the oriTR conjugation test 51 plates were made:
 +
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 1</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D1ori || TRI + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D2ori || TRI + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| D3ori || TRI + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D4ori || TRI + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| D5ori || TRI + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| Ts1ori || AMP + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| Ts2ori || AMP + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| Ts3ori || AMP + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| Ts4ori || AMP + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| Ts5ori || AMP + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| TL1ori || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| TL2ori || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| TL3ori || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| TL4ori || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| TL5ori || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R1ori || AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R2ori || AMP || 10<sup>-1</sup>||
 +
|}
 +
 +
<br><br>
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 2</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D6ori || TRI + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D7ori || TRI + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| D8ori || TRI + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D9ori || TRI + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| D10ori || TRI + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| Ts6ori || AMP + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| Ts7ori || AMP + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| Ts8ori || AMP + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| Ts9ori || AMP + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| Ts10ori || AMP + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| TL6ori || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| TL7ori || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| TL8ori || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| TL9ori || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| TL10ori || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R3ori || AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R4ori || AMP || 10<sup>-1</sup>||
 +
|}
 +
 +
 +
<br><br>
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 3</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D11ori || TRI + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D12ori || TRI + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| D13ori || TRI + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D14ori || TRI + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| D15ori || TRI + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| Ts11ori || AMP + CAM || 10<sup>0</sup> ||
 +
|- align="center"
 +
| Ts12ori || AMP + CAM || 10<sup>-1</sup>||
 +
|- align="center"
 +
| Ts13ori || AMP + CAM || 10<sup>-2</sup>||
 +
|- align="center"
 +
| Ts14ori || AMP + CAM || 10<sup>-3</sup>||
 +
|- align="center"
 +
| Ts15ori || AMP + CAM || 10<sup>-4</sup>||
 +
|- align="center"
 +
| TL11ori || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| TL12ori || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| TL13ori || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| TL14ori || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| TL15ori || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R5ori || AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R6ori || AMP || 10<sup>-1</sup>||
|}
|}
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 18:24, 17 August 2009

Lab Notebook

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18 August 2009

Calin

Yesterdays 20 well 1% gel:


Well Part Expected Plasmid Size Status
1
2
3
4
5
6 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
7 Colony PCR 1A - R751ΔtrbK 1616
8 Colony PCR 1A - R751ΔtrbK 1616
9 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
10 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
11 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398
12 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔

Yesterdays 26 well 2% gel:


Well Part Expected Plasmid Size Status
1 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
20
21
22 Colony PCR 1A - R751ΔtrbK 1616
23 Colony PCR 1A - R751ΔtrbK 1616
24 Colony PCR R751 + pKD46 + trbK_KO_mod_S 348
25 Colony PCR R751ΔoriTR -L-ara +PCR control  ?
26 Colony PCR R751 + pKD46 + oriTR_KO_mod_S 398


For the trbK conjugation test 24 plates were made:


Set 1
Plate ID Antibiotics Dilution
D1tr TRI 100
D2tr TRI 10-1
D3tr TRI 10-2
D4tr TRI 10-3
D5tr TRI 10-4
T1tr TRI + CAM 100
T2tr TRI + CAM 10-1
T3tr TRI + CAM 10-2
T4tr TRI + CAM 10-3
T5tr TRI + CAM 10-4
R1tr CAM 100
R2tr CAM 10-1



Set 2
Plate ID Antibiotics Dilution
D6tr TRI 100
D7tr TRI 10-1
D8tr TRI 10-2
D9tr TRI 10-3
D10tr TRI 10-4
T6tr TRI + CAM 100
T7tr TRI + CAM 10-1
T8tr TRI + CAM 10-2
T9tr TRI + CAM 10-3
T10tr TRI + CAM 10-4
R3tr CAM 100
R4tr CAM 10-1

For the oriTR conjugation test 51 plates were made:

Set 1
Plate ID Antibiotics Dilution
D1ori TRI + CAM 100
D2ori TRI + CAM 10-1
D3ori TRI + CAM 10-2
D4ori TRI + CAM 10-3
D5ori TRI + CAM 10-4
Ts1ori AMP + CAM 100
Ts2ori AMP + CAM 10-1
Ts3ori AMP + CAM 10-2
Ts4ori AMP + CAM 10-3
Ts5ori AMP + CAM 10-4
TL1ori TRI + AMP 100
TL2ori TRI + AMP 10-1
TL3ori TRI + AMP 10-2
TL4ori TRI + AMP 10-3
TL5ori TRI + AMP 10-4
R1ori AMP 100
R2ori AMP 10-1



Set 2
Plate ID Antibiotics Dilution
D6ori TRI + CAM 100
D7ori TRI + CAM 10-1
D8ori TRI + CAM 10-2
D9ori TRI + CAM 10-3
D10ori TRI + CAM 10-4
Ts6ori AMP + CAM 100
Ts7ori AMP + CAM 10-1
Ts8ori AMP + CAM 10-2
Ts9ori AMP + CAM 10-3
Ts10ori AMP + CAM 10-4
TL6ori TRI + AMP 100
TL7ori TRI + AMP 10-1
TL8ori TRI + AMP 10-2
TL9ori TRI + AMP 10-3
TL10ori TRI + AMP 10-4
R3ori AMP 100
R4ori AMP 10-1




Set 3
Plate ID Antibiotics Dilution
D11ori TRI + CAM 100
D12ori TRI + CAM 10-1
D13ori TRI + CAM 10-2
D14ori TRI + CAM 10-3
D15ori TRI + CAM 10-4
Ts11ori AMP + CAM 100
Ts12ori AMP + CAM 10-1
Ts13ori AMP + CAM 10-2
Ts14ori AMP + CAM 10-3
Ts15ori AMP + CAM 10-4
TL11ori TRI + AMP 100
TL12ori TRI + AMP 10-1
TL13ori TRI + AMP 10-2
TL14ori TRI + AMP 10-3
TL15ori TRI + AMP 10-4
R5ori AMP 100
R6ori AMP 10-1

Retrieved from "http://2009.igem.org/Team:TUDelft/18_August_2009"