"
Team:TUDelft/20 August 2009
From 2009.igem.org
(Difference between revisions)
Tim Weenink (Talk | contribs) (→20 August 2009) |
Tim Weenink (Talk | contribs) (→Tim Weenink) |
||
| Line 12: | Line 12: | ||
I inoculated some of the colonies. These are the names of the samples: | I inoculated some of the colonies. These are the names of the samples: | ||
| + | R/E stands for RbCl/Electrocompetent (resp) | ||
| + | 1C3 stands for the used backbone (pSB1C3) | ||
α ß stands for plate numbers | α ß stands for plate numbers | ||
1,2 stands for colony numbers | 1,2 stands for colony numbers | ||
Revision as of 21:42, 20 August 2009
Lab Notebook
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
20 August 2009
Tim Weenink
We got colonies on yesterdays transformations. RbCl plates had about 20 colonies each, while Electrocompetent cells were hundreds of colonies. Finally an oldfashioned highly efficient transformation. I suspect transformations with larger parts (>1kb) work better.
I inoculated some of the colonies. These are the names of the samples:
R/E stands for RbCl/Electrocompetent (resp) 1C3 stands for the used backbone (pSB1C3) α ß stands for plate numbers 1,2 stands for colony numbers
- !SIIR1C3α1
- !SIIR1C3α2
- !SIIR1C3ß1
- !SIIR1C3ß2
- !SIIE1C3α1
- !SIIE1C3α2
- !SIIE1C3ß1
- !SIIE1C3ß2
