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Team:TUDelft/1 September 2009
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Today I leave the lab and I'll be helping in planning and experimental design. | Today I leave the lab and I'll be helping in planning and experimental design. | ||
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| + | ===Tim Weenink=== | ||
| + | Today I restricted p(LacI) as an upstream part and *T1 (homing endonuclease without promotor) as a downstream part. Additionally I ligated the restricted I-SceI PCR fragment. This fragment was PCR'd from the plasmid from leiden containing the conding sequence of ISCEI. It was purified and restricted using E and P. Using the standard protocol, it was ligated into a pSB1A3 vector. | ||
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| + | Finally, Esengul streaked a plate of K12 strain, that allows IPTG induction (without being expressed constitutively). This strain wil be grown up and made electro competent. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 15:34, 1 September 2009
Lab Notebook
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1 September 2009
Daniel
Miniprep of yesterday's cultures:
| Part | [DNA] ng/uL |
| 1 | |
| 1 | |
| 1 | |
| 1 |
As noticed, 1C concentration is low, this is because I add neutralization buffer before lysis buffer. We will do it again tomorrow.Thanks to Esengul, we will have electrocompetent cells which produce LacI.
Today I leave the lab and I'll be helping in planning and experimental design.
Tim Weenink
Today I restricted p(LacI) as an upstream part and *T1 (homing endonuclease without promotor) as a downstream part. Additionally I ligated the restricted I-SceI PCR fragment. This fragment was PCR'd from the plasmid from leiden containing the conding sequence of ISCEI. It was purified and restricted using E and P. Using the standard protocol, it was ligated into a pSB1A3 vector.
Finally, Esengul streaked a plate of K12 strain, that allows IPTG induction (without being expressed constitutively). This strain wil be grown up and made electro competent.
