Team:TUDelft/4 September 2009

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I transformed all four batches of competent cells with !SIII ligation mix. Plates (Cam) were left on the bench in a plastic bag over the weekend. All the time constants in the electroporation were 4.4 or 4.5 (which is good). Also two plates without selection marker were streaked to check if the competent cells were viable at all.
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Revision as of 20:32, 6 September 2009

Lab Notebook

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4 September 2009

Tim Weenink

I transformed all four batches of competent cells with !SIII ligation mix. Plates (Cam) were left on the bench in a plastic bag over the weekend. All the time constants in the electroporation were 4.4 or 4.5 (which is good). Also two plates without selection marker were streaked to check if the competent cells were viable at all.