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Team:Paris
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Revision as of 18:45, 20 October 2009
iGEM > Paris > Home > Synopsis
Contents |
Message in a Bubble
Abstract
Message in a Bubble: a robust inter-cellular communication system based on outer membrane vesicles. Sending a message across the ocean… Outer membrane vesicles (OMV), naturally produced by gram negative bacteria such as E. Coli, are strong candidates for long-distance messaging. Our engineered communication platform consists in controlling OMV production by destabilizing membrane integrity through over-expression of specific periplasmic proteins (e.g. TolR). In order to control and modulate message content, we used fusions with OmpA signal sequence and the ClyA hemolysin as delivery tags. A targeting system was developed, based on the outer-membrane expression of Jun/Fos leucine zippers to control the vesicle flux between donor and recipient cells. Once received, the signal from incoming vesicles is transduced through a modified Fec pathway, whereby the receptor is provided by the OMV. Computational models provided insight to all of the above steps. Such reliable communications systems have wide biotechnological implications, ranging from targeted drugs delivery and detoxification to advanced division of labor or even cell-based computing.
Strategy
We aimed developing a long distance communication system between gram-negative bacteria that is based on the ability of these organisms to produce Outer-Membrane Vesicles (OMVs). We designed a framework which can be easily expanded to a lot of different inputs/ouputs. We hope this standardized approach will increase our capacity to manipulate information and/or exchange it between bacteria. This can be used in any engineered biological process requiring transformation or a system of information transfer, including medical applications and bio-remediation.
We did our best to standardize:
- the increase of vesicle production;
- the system to address proteins to these vesicles;
- their fusion with target bacteria.
You can find detailed information in our OMV Project description or in our different parts.
By analogy to the Internet Protocol, we called our process a Bacterial Protocol. We found that the title Message in a bubble described it quite nicely. In a simple way, the comprehension of vesicles process sounds like a build of a bacteria language.
For more information on how we came up with this idea, please have a look at our brainstorming area.
Here we are ! You have some problems to navigate in our wiki ?? Just look at this page, and we hope it will help you.
And, if your are interested in a guided visit, the super/best/ultimate essential of the iGEM Paris 2009 wiki,..., just follow the white rabbit Open Book...
Extended Team
iGEM Paris Team
Hello everyone! welcome to our wiki. We are a team of 13 students (6 biologists, 2 mathematicians, 1 computer scientist, 1 sociologist, 1 infectiologist, 1 geneticist, and 1 chemist) highly motivated to do our best for the iGEM competition. We decided to put our different knowledge for this scientific and human adventure.
Concerning supervisors, we've got very good scientists/professors like Ariel Lindner, Guillaume Cambray and Samuel Bottani. So... let's the contest begin! (Details on our team here).
Additional information on all iGEM Paris team here : [http://www.igem-paris.org http://www.igem-paris.org]
Our Sponsors
| bettancourt | Mines | Inria | |
| Embassy | |||
| Eco | biolabs |
Collaborations and Visitors
With BCCS-Bristol , we shared our modeling part concerning the production of vesicles which is supposed to be complementary of their and we hope that we will be able to combine the two approach to make one big model of the production , adressing , and fusion of the vesicles.
For more details, see our Collaborations web page.
We have also created a poll concerning the Iphone Software and we sent it to the other Igem team , 4 of them replied,
Find the results and more details on Collaborations page.
All the team answered to the Valencia ethics poll that is why you can see this gorgeous gold medal with our name on it. More seriously it was very interesting to see what all members answered to each questions.
Looking for more details ? see our Collaborations web page.
And we thank all the visitors, without them we couldn't have a so eclectic cluster map.
Thank you Internal Notes
Image très jolie mais qui nécessite une légente pour explique les trois élements: production, vésicule, cible. Peut-être d'ailleurs ici mettre des liens directs vers les parties correspondantes du wiki.
Un élément à introduire dans le wiki et sur lequel je reviendrai de cesse tant qu'il ne sera pas là: des liens de navigation pour une visite guidée, séquentielle du wiki. Qu'il puisse être possible de le parcourir facilement dans un ordre logique suivant votre histoire. Il est trop facile dans les wiikis de se perdre dans les ramifications des branches des différentes pages et de ne ressortir qu'avec une idée confuse du contenu du site.
Les menus de gauche ne sont pas clairs ainsi que l'architecture du site: de grandes parties mélangées à des sous parties: Adressing, Production, Reception ne devraient pas être au même niveau que Home, The Project, Ehics, Tools...
En parcourant l'ensemble je pense que ce serait utile pour la vulgarisation du projet d'avoir une partie "Cell Biology Reminder" qui rappelerait les bases scientifiques nécessaire aiu projet: Gram-negative bacteria, The membrane of a gram-negative bacteria, Veiscles etc...
flefevre : j'ai viré les parties non essentielles dans la page Draft. On peut donc y retrouver les éléments précédemment écrits.
flefevre : je pense qu il faut une page dedie a expliquer comment naviguer dans votre wiki, eventuellement une capture d une page qui explicite les differents menus.
il y a en effet :
le menu en haut a gauche
le menu avec les liens pour savoir la profondeur, ou l'on est
le menu de gauche
le menu de droite
le menu du centre qui change en fonction de la ou l on est
cela fait 5 menus ... sans commentaires
flefevre : je pense qu il faut augmenter la taille en largeur du menu de gauche pour pouvoir avoir des titres de menu long = 2 mots qui veulent dire quelques choses
</html>
A FAIRE
Project Overview = Corriger
Modelling Overview = A faire partie Ch
Conclusion = A faire
Export = Tat vs Sec : schéma
Stratégie = relire
Construction = A faire
ClyA = relire + stratégie : relire +
Wet lab = a finir
Vesicule prod system = traduire; construction = à corriger
Vesicule prod model = corriger
Modeling vesicule creation = cf Peter
Wet lab = pbme de mise en page
Membrane fusion (all parts): Fait (CO) --> à relire
"To the cytoplasm" : Fait (CO) --> à relire
"In the outer membrane", Intro + OmpA : Fait (CO) --> à relire
Strategy + Construction = NADA
Tansduction = a corriger
Fec Simu = cf ch
