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Team:TUDelft/16 September 2009
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Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. | Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. | ||
Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys. | Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys. | ||
| + | Then they were electroporated to cells. | ||
===Tim Weenink=== | ===Tim Weenink=== | ||
Revision as of 00:08, 22 October 2009
Lab Notebook
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16 September 2009
Sriram
Today I checked in gel (see below) that the small colonies of LacI generator and Normal RBS control has the plasmids of correct sizes. Hence with the correct LacI generator I ligated the control plasmids, Plasmid 1 of negative cascade and locks and keys. Then they were electroporated to cells.
Tim Weenink
Ligated the following constructs: !Y and !X and transformed the E. Coli K12 strain with them.
