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Team:TUDelft/27 July 2009
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Placed GFP / RFP plates in fridge. | Placed GFP / RFP plates in fridge. | ||
===Orr=== | ===Orr=== | ||
| - | Used mfold to produce 3 locks: one with weak RBS, one with weaker RBS and one with strong RBS. | + | Used mfold to produce 3 locks: one with weak RBS, one with weaker RBS and one with strong RBS.<br> |
| - | Using the 2-state hybridization server, combined the key3c with each of the 4 locks (lock3c, weak lock, weaker lock and strong lock) to obtain the hybridised structure. | + | Using the 2-state hybridization server, combined the key3c with each of the 4 locks (lock3c, weak lock, weaker lock and strong lock) to obtain the hybridised structure.<br> |
| - | Created weak key, weaker key and strong key sequences for each of the last three locks by taking the hybridization of each lock with the key3c and correcting the kinks by changing the key3c sequence. | + | Created weak key, weaker key and strong key sequences for each of the last three locks by taking the hybridization of each lock with the key3c and correcting the kinks by changing the key3c sequence.<br> |
Biobricked the weak, weaker and strong keys into the registry. | Biobricked the weak, weaker and strong keys into the registry. | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 16:49, 27 July 2009
Lab Notebook
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27 July 2009
Calin
Placed GFP / RFP plates in fridge.
Orr
Used mfold to produce 3 locks: one with weak RBS, one with weaker RBS and one with strong RBS.
Using the 2-state hybridization server, combined the key3c with each of the 4 locks (lock3c, weak lock, weaker lock and strong lock) to obtain the hybridised structure.
Created weak key, weaker key and strong key sequences for each of the last three locks by taking the hybridization of each lock with the key3c and correcting the kinks by changing the key3c sequence.
Biobricked the weak, weaker and strong keys into the registry.
