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Team:Paris/4 August 2009
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*1/1K named 1K1 (pSB1A3). Resistant to Ampicilin. | *1/1K named 1K1 (pSB1A3). Resistant to Ampicilin. | ||
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| + | Transformation of precited Biobrick | ||
| + | </div><div style="background-color:#E0E9EF; border:1px solid #333333; padding:3px 5px; margin-bottom:10px"> | ||
| + | *1K1 (pSB1A3) in competent DH5α by RbCl [[https://2009.igem.org/Team:Paris/Protocols_transformation Protocol]] | ||
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Revision as of 01:00, 5 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
Overnight culture
- Keio Collection
- [S20] JW4251: LB Kan1000x 200rpm 37°C
- [S21] JN0729: LB Kan1000x 200rpm 37°C
- [S22] JN0728: LB Kan1000x 200rpm 37°C
- [S23] JN0727: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 200rpm 37°C
- [S24] JN0940: LB Kan1000x 37°C
- [S25] JW5100: LB Kan1000x 200rpm 37°C
- [S25] JW5100: LB Kan1000x 37°C
- [S26] JN5181: LB Kan1000x 200rpm 37°C
- [S27] FF64: LB Tet1000x 200rpm 37°C
- [S28] NEC280: LB 200rpm 37°C
- [S5] MG4: LB Kan NaCl20% 200rpm 37°C
- [S6] DH5α: LB 200rpm 37°C
- [S24] JN0940: LBagar plate with Kan1000x 37°C
- [S25] JW5100: LBagar plate with Kan1000x 37°C
Biobrick resuspention
- 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
Transformation of precited Biobrick
- 1K1 (pSB1A3) in competent DH5α by RbCl [Protocol]
To do list
| Matricule | TODO |
| Luc | SNARE Construction |
| Romain | Design oligo Fec A |
| Charlotte | design of oligos: FecR, FecA, SNARE?, sequencing oligo |
| Stoff | Dev schedule algorithm / BDD |
| Chris | modeling : we don't want fu*king oscillations ! |
| Lisa | Oligo Tol/Pal / Oligo Sequencing / Tol/Pal Protocol verification / Keio / Theme A writting |
| Caroline | coloration of cells / protocols for the lab: control of the emission of vesicules.... |
| Souf | oligos desing / Labs |
| Vicard | Gel / SNARE |
| Pierre | modeling : we don't want fu*king oscillations ! |
| Sylvain | minipreps. Check if the use of starch is possible ; problem : find a system to help the starch enter into the periplasm, use of LacY -> noise |
| Guillaume | Lab/ g3p |
