Team:Paris/Freezer Digestion Products
From 2009.igem.org
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Revision as of 14:52, 5 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
Digestion Products | |||||||||
---|---|---|---|---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enz. 1 | Enz. 2 | Size | Description | Product Left | []ng/µL | Date |
D1 | P3 | BBa-J32015 | XbaI | PstI | Size | PelB leader sequence | ??? | ??? | 08/04 |
D2 | P4 | BBa-B0014 | XbaI | PstI | Size | Double terminator bidirectionnal (B0012-B0011) | ??? | ??? | 08/04 |
D3 | P5 | BBa-I14032 | XbaI | PstI | Size | pLac | ??? | ??? | 08/04 |
D4 | P7 | Bba-R0040 | XbaI | PstI | Size | TetR repressible promote | ??? | ??? | 08/04 |
D5 | P8 | BBa-I0500 | XbaI | PstI | Size | pBad/araC | ??? | ??? | 08/04 |
D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | ??? | ??? | 08/04 |
D7 | ??? | ??? | XbaI | PstI | Size | ??? | ??? | ??? | 08/04 |