Team:Paris/Freezer PCR products

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|width=5%| []ng/µL
|width=5%| []ng/µL
|width=5%| Date
|width=5%| Date
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A1
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|width=10%| ?
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|width=10%| [O18] Tg3p_fusion_Fw 
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|width=10%| [O17] Tg3p_Rw
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|width=5%| ?
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|width=10%| ?
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|width=10%| 274bp
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|width=25%| Amplification of N-term domain of M13's g3p
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|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A2
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|width=10%| ?
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|width=10%| [O15] TE3_fusion_Fw
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|width=10%| [O16] TE3_Rw
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|width=5%| ?
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|width=10%| ?
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|width=10%| 1036bp
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|width=25%| Amplification of N-term domain of colicin E3
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|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A3
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|width=10%| ?
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|width=10%| [O31] clyA_RBS_Fw
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|width=10%| [O10] clyA_fusion_polyG_Rw
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|width=5%| ?
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|width=10%| ?
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|width=10%| 987bp
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|width=25%| Amplification of clyA with RBS in the primer
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|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A4
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|width=10%| ?
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|width=10%| [O11] ompA_signal_Fw
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|width=10%| [O12] ompA_signal_fusion_Rw
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|width=5%| ?
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|width=10%| ?
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|width=10%| 135bp
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|width=25%| Amplification of OmpA signal for export to periplasm
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|width=5%| ?
 +
|width=5%| ? ng/µL
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|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A5
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|width=10%| ?
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|width=10%| [O1] Rev P1
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|width=10%| [O13] tolR_II_Fusion_Fw
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|width=5%| ?
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|width=10%| ?
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|width=10%| 279bp
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|width=25%| Amplification of tolR
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|width=5%| ?
 +
|width=5%| ? ng/µL
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|width=5%| ?
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|- style="background: #ccccff; text-align: center;"
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|width=5%| A6
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|width=10%| ?
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|width=10%| [O14] tatABC_RBS_Fw
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|width=10%| [O5] Rev P5
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|width=5%| ?
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|width=10%| ?
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|width=10%| 1634bp
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|width=25%| Amplification of tatA with RBS
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|width=5%| ?
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|width=5%| ? ng/µL
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|width=5%| ?
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|width=5%| A?
|width=5%| A?

Revision as of 19:27, 5 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Number Name Oligo F Oligo R Tm Matrix Length Comments Quantity []ng/µL Date
A1  ? [O18] Tg3p_fusion_Fw [O17] Tg3p_Rw  ?  ? 274bp Amplification of N-term domain of M13's g3p  ?  ? ng/µL  ?
A2  ? [O15] TE3_fusion_Fw [O16] TE3_Rw  ?  ? 1036bp Amplification of N-term domain of colicin E3  ?  ? ng/µL  ?
A3  ? [O31] clyA_RBS_Fw [O10] clyA_fusion_polyG_Rw  ?  ? 987bp Amplification of clyA with RBS in the primer  ?  ? ng/µL  ?
A4  ? [O11] ompA_signal_Fw [O12] ompA_signal_fusion_Rw  ?  ? 135bp Amplification of OmpA signal for export to periplasm  ?  ? ng/µL  ?
A5  ? [O1] Rev P1 [O13] tolR_II_Fusion_Fw  ?  ? 279bp Amplification of tolR  ?  ? ng/µL  ?
A6  ? [O14] tatABC_RBS_Fw [O5] Rev P5  ?  ? 1634bp Amplification of tatA with RBS  ?  ? ng/µL  ?
A? g3p_std [O19] g3p_F [O20] g3p_R 78°C P12 1287bp For standardisation of g3p and adding SacI site  ?  ? ng/µL  ?
A? OmpA_L_std [O21] Ompa_linker_F [O22] Ompa_linker_R 80°C P6 525bp For standardisation of OmpA linker (BBaK103006)  ?  ? ng/µL  ?



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png

http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

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