Team:TUDelft/5 August 2009

From 2009.igem.org

(Difference between revisions)
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===Tim Weenink===
===Tim Weenink===
-
[[Image:Tim050809.png|thumb|]]
+
[[Image:Tim050809.png|400px|]]
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===Orr===
===Orr===
Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.
Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.
 +
 +
===Calin===
 +
 +
Made test plate for Trimpethoprim.
 +
 +
Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.
 +
 +
Ran three conjugations in parallel, with a conjugation time of 1 hour. Made a total of 40 plates:
 +
 +
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 1</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D1 || TRI || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D2 || TRI || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D3 || TRI || 10<sup>-4</sup>||
 +
|- align="center"
 +
| D4 || TRI || 10<sup>-5</sup>||
 +
|- align="center"
 +
| D5 || TRI || 10<sup>-6</sup>||
 +
|- align="center"
 +
| T1 || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| T2 || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| T3 || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| T4 || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| T5 || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| T6 || TRI + AMP || 10<sup>-5</sup> ||
 +
|- align="center"
 +
| T7 || TRI + AMP || 10<sup>-6</sup>||
 +
|- align="center"
 +
| R1 || AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| R2 || AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| R3 || AMP || 10<sup>-5</sup>||
 +
|- align="center"
 +
| R4 || AMP || 10<sup>-6</sup>||
 +
|}
 +
 +
<br><br>
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 2</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D6 || TRI || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D7 || TRI || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D8 || TRI || 10<sup>-4</sup>||
 +
|- align="center"
 +
| D9 || TRI || 10<sup>-5</sup>||
 +
|- align="center"
 +
| D10 || TRI || 10<sup>-6</sup>||
 +
|- align="center"
 +
| T8 || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| T9 || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| T10 || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| T11 || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| T12 || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| T13 || TRI + AMP || 10<sup>-5</sup> ||
 +
|- align="center"
 +
| T14 || TRI + AMP || 10<sup>-6</sup>||
 +
|}
 +
 +
<br><br>
 +
{| border="1" align="center"
 +
|-
 +
| <b>Set 3</b>
 +
|- align="center"
 +
| Plate ID || Antibiotics || Dilution ||
 +
|- align="center"
 +
| D11 || TRI || 10<sup>0</sup> ||
 +
|- align="center"
 +
| D12 || TRI || 10<sup>-2</sup>||
 +
|- align="center"
 +
| D13 || TRI || 10<sup>-4</sup>||
 +
|- align="center"
 +
| D14 || TRI || 10<sup>-5</sup>||
 +
|- align="center"
 +
| D15 || TRI || 10<sup>-6</sup>||
 +
|- align="center"
 +
| T15 || TRI + AMP || 10<sup>0</sup> ||
 +
|- align="center"
 +
| T16 || TRI + AMP || 10<sup>-1</sup>||
 +
|- align="center"
 +
| T17 || TRI + AMP || 10<sup>-2</sup>||
 +
|- align="center"
 +
| T18 || TRI + AMP || 10<sup>-3</sup>||
 +
|- align="center"
 +
| T19 || TRI + AMP || 10<sup>-4</sup>||
 +
|- align="center"
 +
| T20 || TRI + AMP || 10<sup>-5</sup> ||
 +
|- align="center"
 +
| T21 || TRI + AMP || 10<sup>-6</sup>||
 +
|}
{{Template:TUDelftiGEM2009_end}}
{{Template:TUDelftiGEM2009_end}}

Revision as of 11:15, 6 August 2009

Lab Notebook

July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

5 August 2009

Tim Weenink

Tim050809.png


Tim050809!Dandcalin.png


Well Part Expected Plasmid Size Status
1
2
3
4
5
6
7
8
9 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
10 pSB1AK3 linear 3864 ✔
11 pSB4C5 linear 3896 ✔
12 strong promoter 2983 ✔
13 oriT-R linear  ? ✔

Orr

Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.

Calin

Made test plate for Trimpethoprim.

Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.

Ran three conjugations in parallel, with a conjugation time of 1 hour. Made a total of 40 plates:


Set 1
Plate ID Antibiotics Dilution
D1 TRI 100
D2 TRI 10-2
D3 TRI 10-4
D4 TRI 10-5
D5 TRI 10-6
T1 TRI + AMP 100
T2 TRI + AMP 10-1
T3 TRI + AMP 10-2
T4 TRI + AMP 10-3
T5 TRI + AMP 10-4
T6 TRI + AMP 10-5
T7 TRI + AMP 10-6
R1 AMP 100
R2 AMP 10-4
R3 AMP 10-5
R4 AMP 10-6



Set 2
Plate ID Antibiotics Dilution
D6 TRI 100
D7 TRI 10-2
D8 TRI 10-4
D9 TRI 10-5
D10 TRI 10-6
T8 TRI + AMP 100
T9 TRI + AMP 10-1
T10 TRI + AMP 10-2
T11 TRI + AMP 10-3
T12 TRI + AMP 10-4
T13 TRI + AMP 10-5
T14 TRI + AMP 10-6



Set 3
Plate ID Antibiotics Dilution
D11 TRI 100
D12 TRI 10-2
D13 TRI 10-4
D14 TRI 10-5
D15 TRI 10-6
T15 TRI + AMP 100
T16 TRI + AMP 10-1
T17 TRI + AMP 10-2
T18 TRI + AMP 10-3
T19 TRI + AMP 10-4
T20 TRI + AMP 10-5
T21 TRI + AMP 10-6

Retrieved from "http://2009.igem.org/Team:TUDelft/5_August_2009"