Team:TUDelft/5 August 2009

From 2009.igem.org

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Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.
Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.
-
Ran three conjugations in parallel, with a conjugation time of 1 hour. Made a total of 40 plates:
+
Ran three conjugations in parallel, with a conjugation time of 1 hour.  
 +
 
 +
Starting OD for recipients 0.502, for donors 0.542.
 +
 
 +
Made a total of 40 plates:

Revision as of 18:55, 6 August 2009

Lab Notebook

July
MTWTFSS
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6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
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7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
 

5 August 2009

Tim Weenink

Tim050809.png


Tim050809!Dandcalin.png


Well Part Expected Plasmid Size Status
1
2
3
4
5
6
7
8
9 [http://www.eurogentec.com/EGT/Images/RESALES/Electrophoresis/Regular%20DNA%20Ladder/7-SmartLadder.jpg DNA Ladder] ✔
10 pSB1AK3 linear 3864 ✔
11 pSB4C5 linear 3896 ✔
12 strong promoter 2983 ✔
13 oriT-R linear  ? ✔

Orr

Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.

Calin

Made test plate for Trimpethoprim.

Ran conjugation test with R751 (TRI) as donor and DH5 α cells with GFP plasmid and AMP as recipients.

Ran three conjugations in parallel, with a conjugation time of 1 hour.

Starting OD for recipients 0.502, for donors 0.542.

Made a total of 40 plates:


Set 1
Plate ID Antibiotics Dilution
D1 TRI 100
D2 TRI 10-2
D3 TRI 10-4
D4 TRI 10-5
D5 TRI 10-6
T1 TRI + AMP 100
T2 TRI + AMP 10-1
T3 TRI + AMP 10-2
T4 TRI + AMP 10-3
T5 TRI + AMP 10-4
T6 TRI + AMP 10-5
T7 TRI + AMP 10-6
R1 AMP 100
R2 AMP 10-4
R3 AMP 10-5
R4 AMP 10-6



Set 2
Plate ID Antibiotics Dilution
D6 TRI 100
D7 TRI 10-2
D8 TRI 10-4
D9 TRI 10-5
D10 TRI 10-6
T8 TRI + AMP 100
T9 TRI + AMP 10-1
T10 TRI + AMP 10-2
T11 TRI + AMP 10-3
T12 TRI + AMP 10-4
T13 TRI + AMP 10-5
T14 TRI + AMP 10-6



Set 3
Plate ID Antibiotics Dilution
D11 TRI 100
D12 TRI 10-2
D13 TRI 10-4
D14 TRI 10-5
D15 TRI 10-6
T15 TRI + AMP 100
T16 TRI + AMP 10-1
T17 TRI + AMP 10-2
T18 TRI + AMP 10-3
T19 TRI + AMP 10-4
T20 TRI + AMP 10-5
T21 TRI + AMP 10-6

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