Team:Paris/6 August 2009
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protocol n°2 (05/08/09)but 1ml of cells and 1µl of dye, one with MgSO4 and one with PBS | protocol n°2 (05/08/09)but 1ml of cells and 1µl of dye, one with MgSO4 and one with PBS | ||
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Revision as of 08:17, 7 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
FM4-64 dying MG4 and Kayo
protocol n°2 (05/08/09) but using PBS
->nearly the same as MgSO4
protocol n°2 (05/08/09)but 1ml of cells and 1µl of dye, one with MgSO4 and one with PBS
->not enough dye
Writing tomorow...
- Nouvelles Miniprep pour P1(pSB2K3), P13(pSB1A3), P14(BBa_K136050) => Nulles à refaire pousser ON et miniprep a refaire demain
- ON pour S8(pSB2K3), S29(pSB1A3), S30(BBa_K136050)
- Digestion P1(pSB2K3), P2(BBa_J61002), P7(Bba_R0040) 2h37 par respectivement XP, XS, XP
- Migration sur gel pour verifier 1kb, P1, P2, P7, 100bp
- Transformation 1L7 BBa_J24679 AmpR+ dans DH5a
To do list
Matricule | TODO |
Luc | Labs |
Romain | Look for his feet |
Charlotte | Jun/Fos system (oligo + system) |
Stoff | DataBase / kitchen system ? |
Chris | modeling: model genetique network / next week lab planning / gillepsy |
Lisa | WTF !!! |
Caroline | lab / microscope |
Souf | lab / oligo / wiki |
Vicard | Lab : Gel :D |
Pierre | Tol/pal modeling |
Sylvain | if(!oligo){return Maltose;} return PCR; |
Guillaume | ?? |