Team:Paris/Freezer Digestion Products
From 2009.igem.org
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|- style="background: #0d3e99; text-align: center; color:white;" | |- style="background: #0d3e99; text-align: center; color:white;" | ||
|width=5%| Number | |width=5%| Number | ||
- | |width=20%| | + | |width=20%| Name |
- | |width=20%| Matrix | + | |width=20%| Description |
+ | |width=20%| Matrix ID | ||
|width=5%| Enz. 1 | |width=5%| Enz. 1 | ||
|width=5%| Enz. 2 | |width=5%| Enz. 2 | ||
- | |width=5%| Size | + | |width=5%| Size (bp) |
- | |width= | + | |width=10%| Digested? |
- | |width=10%| | + | |width=10%| Purified? |
- | |width=5%| [ | + | |width=5%| [ng/µL] |
- | + | ||
|- style="background: #ccccff; text-align: center;" | |- style="background: #ccccff; text-align: center;" | ||
|style="background:#FFFF00"| D1 | |style="background:#FFFF00"| D1 |
Revision as of 12:57, 7 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
Color code for sticker | |||||||
---|---|---|---|---|---|---|---|
Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
Digestion Products | |||||||||
---|---|---|---|---|---|---|---|---|---|
Number | Name | Description | Matrix ID | Enz. 1 | Enz. 2 | Size (bp) | Digested? | Purified? | [ng/µL] |
D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | 66pb | PelB leader sequence | 47 | ??? | 08/04 |
D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | 95pb | Double terminator bidirectionnal (B0012-B0011) | 47 | ??? | 08/04 |
D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | 37pb | pLac | 47 | ??? | 08/04 |
D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | 54pb | TetR repressible promote | 47 | ??? | 08/04 |
D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | 1210pb | pBad/araC | 47 | ??? | 08/04 |
D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | 47 | ??? | 08/04 |
D7 | ??? | ??? | XbaI | PstI | Size | ??? | ??? | ??? | 08/04 |