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Team:Paris/Freezer Primers
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<center> | <center> | ||
| - | [[Team:Paris/Freezer_Primers | Primers]] - | + | [[Team:Paris/Freezer_Primers#Freezer | Primers]] - |
| - | [[Team:Paris/Freezer_PCR_products | PCR products]] - | + | [[Team:Paris/Freezer_PCR_products#Freezer | PCR products]] - |
| - | [[Team:Paris/Freezer_Plasmids | Plasmids]] - | + | [[Team:Paris/Freezer_Plasmids#Freezer | Plasmids]] - |
| - | [[Team:Paris/Freezer_Digestion_Products | Digestion Products]] - | + | [[Team:Paris/Freezer_Digestion_Products#Freezer | Digestion Products]] - |
| - | [[Team:Paris/Freezer_Unexpected_digestion_results | Unexpected digestion results]] - | + | [[Team:Paris/Freezer_Unexpected_digestion_results#Freezer | Unexpected digestion results]] - |
| - | [[Team:Paris/Freezer_Strains_(Glycerol stock) | Strains (Glycerol stock)]] | + | [[Team:Paris/Freezer_Strains_(Glycerol stock)#Freezer | Strains (Glycerol stock)]] |
</center> | </center> | ||
<br> | <br> | ||
Revision as of 17:20, 7 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Primers
| [O1] Rev P1 | |||||
|---|---|---|---|---|---|
| Description | tolR | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTAGTAAGGCACATCTTTTGCGCCACCG | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O2] Fwd P2 | |||||
|---|---|---|---|---|---|
| Description | tolR (DO NOT USE ERROR) | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGATGAAATCAACATTGTACCGTTGCTGGACGTACTG | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O3] Rev P3 | |||||
|---|---|---|---|---|---|
| Description | tolA | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTACGGTTTGAAGTCCAATGGCG | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O4] Rev P4 | |||||
|---|---|---|---|---|---|
| Description | tolA (ERROR: Not designed for N-term fusion) | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTAATACTAAAAACAATGGCGC | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O5] Rev P5 | |||||
|---|---|---|---|---|---|
| Description | tatABC | ||||
| Sequence (5' > 3') | TCTGCTGCAGCGGCCGCTACTAGTATTATTATTCTTCAGTTTTTTCGCTTTCTGC | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [06] Fwd P6 | |||||
|---|---|---|---|---|---|
| Description | tatABC (without RBS) | ||||
| Sequence (5' > 3') | TCTGGAATTCGCGGCCGCTTCTAGAATGGGTGGTATCAGTATTTGG | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O7] Rev P7 | |||||
|---|---|---|---|---|---|
| Description | clyA fusion | ||||
| Sequence (5' > 3') | TTTCTGCAGCGGCCGCTACTAGTGACTTCAGGTACCTCAAAGAGTGTC | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O8] Fwd P8 | |||||
|---|---|---|---|---|---|
| Description | clyA fusion (ERROR: No poly-Gly linker) | ||||
| Sequence (5' > 3') | TTTGAATTCGCGGCCGCTTCTAGAATGACTGAAATCGTTGCAGATAAAA | ||||
| Designer | D. Bikard | ||||
| Worked | |||||
| [O9] clyA_Rw | |||||
|---|---|---|---|---|---|
| Description | clyA | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGACTTCAGGTACCTCAAAGAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O10] clyA_fusion_polyG_Rw | |||||
|---|---|---|---|---|---|
| Description | clyA fusion (with 5-Gly linker) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGCCGCCGCCGCCGCCGACTTCAGGTACCTCAAAGAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O11] ompA_signal_Fw | |||||
|---|---|---|---|---|---|
| Description | ompA signal for export to periplasm - from expression vector RBS as in pIN-III-ompA-2 vector - keeps vector original RBS | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAACGAGGGCAAAAAATGAAAAAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O12] ompA_signal_fusion_Rw | |||||
|---|---|---|---|---|---|
| Description | ompA signal for export to periplasm - from expression vector pIN-III-ompA-2 - original RBS from the vector | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGCCTGCGCTACGGTAGCGAAAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O13] tolR_II_Fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | For fusion of tolR domain II (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGTCGATCTGCCAGACGCTAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O14] tatABC_RBS_Fw | |||||
|---|---|---|---|---|---|
| Description | Amplification from tatA - contains RBS | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGATGGGTGGTATCAGTATTTGG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O15] TE3_fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | Fusion of N-term domain of colicin E3 (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAGCGGTGGCGATGGACGCGGCCATAAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O16] TE3_Rw | |||||
|---|---|---|---|---|---|
| Description | Amplification of N-term domain of colicin E3 (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGCGTTCATAATTTCGCTCAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O17] Tg3p_Rw | |||||
|---|---|---|---|---|---|
| Description | Amplification of N-term domain of M13's g3p | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAACACTGAGTTTCGTCACCAGTAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O18] Tg3p_fusion_Fw | |||||
|---|---|---|---|---|---|
| Description | Fusion of N-term domain of M13's g3p (standard 23) | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAAAAAAATTATTATTCGCAATTCCTTTAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O19] g3p_F | |||||
|---|---|---|---|---|---|
| Description | g3p | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTCCGCTGAAACTGTTGAAAGTTG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O20] g3p_R | |||||
|---|---|---|---|---|---|
| Description | g3p | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGACTCCTTATTACGCAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O21] Ompa_linker_F | |||||
|---|---|---|---|---|---|
| Description | For standardisation of OmpA linker (BBaK103006) | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGAAAGCTACTAAACTGGTACTGG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O22] Ompa_linker_R | |||||
|---|---|---|---|---|---|
| Description | For standardisation of OmpA linker (BBaK103006) | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGAGCTCCCTCCTCCAGAACC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O23] OmpAsignal-TE3_seq_Fw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | CACAAATAGCGAAAGATGAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O24] OmpAsignal-TE3_seq_Rw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | ATCATCTGCGGGTAATGATG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O25] VAMP2_Seq_Fw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | ACTCACTATAGGGAGACCACA | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O26] VAMP2_Seq_Rv | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | GGGCTTTGTTAGCAGCCGGAT | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O27] ClyA_seq_Fw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | GCAGCTATTTCCAGTCACAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O28] ClyA_seq_Rw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | CGCCCGCAGCAATAGAATAG | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O29] PFecA_Fw | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACGCGGTACTGGATAAACATTTC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O30] PFecA_Rv | |||||
|---|---|---|---|---|---|
| Description | ??? | ||||
| Sequence (5' > 3') | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTTTTGTTGTGTTCAGCTATGAGT | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
| [O31] clyA_RBS_Fw | |||||
|---|---|---|---|---|---|
| Description | amplify clyA with RBS in the primer (std 10) | ||||
| Sequence (5' > 3') | TTGAATTCGCGGCCGCTTCTAGAAGGAGGTATATAATGACTGAAATCGTTGCAGATAAAAC | ||||
| Designer | G. Cambray | ||||
| Not Tested | |||||
